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| Description |  Thematrixmetalloproteinases(MMPs)arealargefamilyof zincendopeptidases.AllMMPsaresynthesizedasinactiveproenzymes.TheactivationoftheseproenzymesisacriticalstepthatleadstodegradationofextracellularmatrixcomponentssuchasfibronectinandcollagentypeIII.MMP14isexpressedinstromalcellsofcolon,breast,andheadandneckandactivatesMMP2(progelatinaseA)bycleavage. |
| BatchNo. | Seeproductlabel |
| Unitsize | 100µg |
| Antigen | Asyntheticpeptide(FFRRHGTPRRLLYCQRS)correspondingtoaregion(561-577)fromhumanMatrixmetalloproteinase-14.Toenhancetheimmunologicalresponse,thispeptidewascoupledtocarrierproteinBSA. |
| OtherNames | MMP-14;EC3.4.24.80;Membrane-typematrixmetalloproteinase1;MT-MMP1;MTMMP1;Membrane-type-1matrixmetalloproteinase;MT1-MMP;MT1MMP;MMP-X1;MMP14; |
| Accession | P50281MMP14_HUMAN; |
| Producedin | Rabbit |
| Purity | Affinitypurifiedonantigencolumn |
| Applications | Immunohistochemistry(IHC)andWesternBlotting(WB).Aconcentrationof0.1-0.5µg/mlisrecommendedforWB.HumanMMP14hasapredictedlengthof582residuesandMWof66kDa.Aconcentrationof0.5-1.0µg/mlisrecommendedtodetecttheproteininformalinfixedandparaffinembeddedtissuesaswellasformalinoracetonefixedfrozentissues.Heatmediatedantigenretrievalisrequired.Aconcentrationof0.5-1.0µg/mlisrecommendedforIHC-F.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWBandIHCagainsttheantigen. |
| Cross-reactivity | Human;rat;expectedtoreactwithmouseduetosequencehomology |
| Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample. |
| Form | Lyophilisedwith5mgBSA,0.9mgNaCl,0.2mgNa2HPO4,0.05mgThimerosal,0.05mgNaN3 |
| Reconstitution | Reconstitutein100µlofsteriledistilledwatertoachieveanantibodyconcentrationof1mg/ml.Centrifugetoremoveanyinsolublematerial. |
| Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles |
| ExpiryDate | 12monthsafterpurchase |
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