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Finda2platesizeofthisproducthere.Enquireaboutbulkdiscountsfortenormoreplates,atsales@Biosensis.com| Description | ThemouseNT3KitisasandwichELISA.ThecaptureantibodyisamonoclonalNT3antibodypre-coatedontothe96-wellstripplatesprovidedinthekit.MousetestsamplesandstandardsofknownNT3concentrationareaddedtothesewellsandallowedtocomplexwiththeboundNT3antibody.AbiotinylatedNT3polyclonalantibodyisthenadded.Thisdetectionantibodybindstotheantigenthuscompletingthesandwich.Afterwashing,anenzymeAvidin-Biotin-Peroxidasecomplex(ABC)isaddedwhichbindstothesecondantibody.TheperoxidasesubstrateTMBisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofNT3presentinthesamples.Thepurposeofthiskitisthein-vitroquantitativedeterminationofmouseNT3insamplessuchasseraandcellculturesupernates.Thiskithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures. BatchNo. | Seeproductlabels | Antigen | NT3isamemberoftheneurotrophinfamily,thatcontrolssurvivalanddifferentiationofvisceralandproprioceptivesensoryneurons.NT3iscloselyrelatedtobothNGFandBDNF.Itmaybeinvolvedinthemaintenanceoftheadultnervoussystem,andmayaffectdevelopmentofneuronsintheembryowhenitisexpressedinhumanplacenta.NT3-deficientmicegeneratedbygenetargetingdisplayseveremovementdefectsofthelimbs.Thematurepeptideofthisproteinisidenticalinallmammalsexaminedincludinghuman,pig,ratandmouse.SUBCELLULARLOCATION:Secretedprotein.TISSUESPECIFICITY:Brainandperipheraltissues.SIMILARITY:BelongstotheNGF-betafamily. | OtherNames | NT-3;NTF3;Neurotrophin-3;HDNF;Nervegrowthfactor2;NGF-2; | Accession | P20181NTF3_MOUSE; | Specificity | Human;Mouse;Rat; | Cross-reactivity | Lessthan2.5%crossreactivitywithNT-4.Nodetectablereactivitywithotherrelevantproteins. | Storage | Storeat4°C | SpecificReferences | DavideD"Amico,ThomasGener,MariaMartínezdeLagrán,MariaVSanchez-Vives,MónicaSantos,MaraDierssen(2016)InfralimbicNeurotrophin-3InfusionRescuesFearExtinctionImpairmentinaMouseModelofPathologicalFear.Neuropsychopharmacology.[Epubaheadofprint]doi:10.1016/j.neulet.2015.12.016. | SandraPetzold,BabetteSommer,AndreaKröber,RobertNitsch,HerbertSchwegler,JohannesVogt,ThomasRoskoden(2015)NT-3proteinlevelsareenhancedinthehippocampusofPRG1-deficientmicebutremainunchangedinPRG1/LPA2doublemutants.NeuroscienceLetters.612:145-8doi:10.1016/j.neulet.2015.12.016. Kitcomponents | TheELISAkitboxcontains1x96pre-coatedstripplates,proteinstandards,detectionreagents,substratebufferandpreciseinstructions. | Range | 15.6pg/ml-1,000pg/ml | Sensitivity | < 2 pg/ml | Kitprotocol |  BEK-2079-1P_as_at_Oct2016_protocol.pdf
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