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| Description | The human Platelet endothelial cell adhesion molecule or CD31 Kit is a sandwich ELISA. The capture antibody is a polyclonal human CD31 antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known CD31 concentration are added to these wells and allowed to complex with the bound CD31 antibody. A biotinylated human polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD31 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human CD31 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures. |
| Batch No. | See product labels |
| Other Names | PECAM-1; CD-31; EndoCAM; GPIIA; PECA1; CD31; PECAM1; |
| Accession | P16284 PECA1_HUMAN; |
| Specificity | Platelet endothelial cell adhesion molecule |
| Storage | Store at 4°C |
| Kit components | The ELISA kit box contains 2 x 96 pre-coated strip plates, protein standards, detection reagents, substrate buffer and precise instructions. |
| Range | 156 pg/ml - 10,000 pg/ml |
| Sensitivity | < 10 pg/ml |
| Kit protocol | Please contact us at biospeak@biosensis.com for a detailed protocol for this ELISA kit. |
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