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| Description | Cyclin-dependentkinaseinhibitorp27(p27KIP1)iscellcycleregulatorymitoticinhibitorofcdkactivityandanimportantregulatorofcellcycleprogression. |
| BatchNo. | Seeproductlabel |
| Unitsize | 100µg |
| Antigen | Recombinantfulllengthrodentp27KIP1protein |
| Clone | IMD-27 |
| OtherNames | Cyclin-dependentkinaseinhibitor1B;Cyclin-dependentkinaseinhibitorp27;p27Kip1;CDKN1B;KIP1; |
| Accession | P46527CDN1B_HUMAN;P46414CDN1B_MOUSE; |
| Producedin | Mouse |
| Purity | IgG |
| Applications | WesternBlotting(WB).Aconcentrationof2.0-4.0µg/mlisrecommendedforWB.Humanp27KIP1hasapredictedlengthof198residuesandMWof22kDa.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWBagainsttheantigen. |
| Cross-reactivity | Human;mouse;rat; |
| Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample. |
| Form | Lyophilizedfrom1.2%sodiumacetate,2mgBSA,0.01mgNaN3 |
| Reconstitution | Reconstitutein1mlofPBS(pH7.4)toachieveanantibodyconcentrationof100µg/ml.Centrifugetoremoveanyinsolublematerial. |
| Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles |
| ExpiryDate | 12monthsafterpurchase |
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