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| Description | Actinisoneofthemostabundantandhighlyconservedproteinsofeukaryotes.Mammalianactinsaretheproductofsixdifferentgeneswithdifferingdistributionpatternsincelltypesandintissues.Themolecularweightofallsixproteinsis42kDa,andoneormoreactinsisfoundinessentiallyeverytypeofcrudecellularandtissueextract.Asaresultantibodiestoactinarewidelyusedasinwesternblottingstandards.Thesecanbeusedtoverifythatthevariousstepsofthewesternblottingprocedurehavebeenperformedcorrectly.Inaddition,actinisregardedasa"housekeeping"proteinwhichisgenerallynotalteredmuchinexpressionasaresultofexperimentalmanipulations.Soquantitationoftheactinbandonthewesternisusedasastandardagainstwiththebanddensityofotherproteinscanbecompared.Themonoclonalbindsallsixactinisotypes(ACTA1,ACTA2,ACTC1,ACTB,ACTG1andACTG2)verystronglyonwesternblots.Itisaveryeffectiveblottingstandardwhichcanworkonanycelltypeortissueextract.Italsoworksinimmunocytochemicalexperiments,bindingstronglyandcleanlytofilopodia,membranerufflesandstressfibers,allknowntoberichinactin. |
| Antigen | Actinpreparedfrombovinebrain. |
| AntibodyType | Monoclonal |
| Isotype | IgG1 |
| Producedin | Mouse |
| Applications | WesternBlotting(WB),Immunocytochemistry(IC)andFlowcytometry.Adilutionof1:5,000-1:10,000isrecommendedforWB.Adilutionof1:500-1:1,000isrecommendedforIC.Use2ug/10^6cellsforFlowcytometry.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | Theantibodyreactswitha42kDabandbyWesternblotonacrudeextractfromHeLacells.Ithasalsobeenusedsuccessfullyforimmunocytochemistry. |
| SpeciesAgainst | Human,rat,mouse,porcineandbovine.Itisexpectedthatitwillworkonothermammaltissues. |
| AntibodyAgainst | Actin |
| Form | LyophilizedfromPBS.Contains5%trehalose. |
| Appearance | Whitepowder |
| Reconstitution | Reconstituteinsteriledistilledwater.Centrifugetoremoveanyinsolublematerial. |
| Storage | Afterreconstitutionoflyophilizedantibody,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
| ExpiryDate | 12monthsafterpurchase |
| GeneralReferences | 1.Vandekerckhove,J.andWeber,K.Atleastsixdifferentactinsareexpressedinahighermammal:ananalysisbasedontheaminoacid.sequenceoftheamino-terminaltrypticpeptide.J.Mol.Biol.126:783802(1978). |
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