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| Description | TheT-cellreceptor-CD3complex(CD3-TCR)playsanimportantroleinmediatingsignalingtransduction.TheCD3-TCRcomplexconsistsofeitheraTCRalpha/betaorTCRgamma/deltaheterodimerco-expressedatthecellsurfacewiththeCD3subunits:CD3gamma,CD3delta,CD3epsilonandCD3zeta.Thesesubunitsaresingle-passtypeImembraneproteinsexpressedonnormalperipheralbloodlymphocytes,thymocytesandPurkinjecellsinthecerebellum. |
| BatchNo. | Seeproductlabel |
| Unitsize | 100µg |
| Antigen | HumanthymocytesfollowedbySezaryTcells |
| Clone | CA-3 |
| OtherNames | T-cellsurfaceglycoproteinCD3;CD3E;CD3G;CD3H;CD3Q;CD3Z;TcellreceptorT3; |
| Accession | P04234CD3D_HUMAN;P07766CD3E_HUMAN; |
| Producedin | Mouse |
| Purity | IgG |
| Applications | Immunohistochemistry(IHC-Frozen).Aconcentrationof1.0µg/mlisrecommendedtodetectCD3informalin/acetonefixedtissues.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | Thespecificityofthisantibodyhasbeenconfirmedbyimmunohistochemistryagainsttheantigen. |
| Cross-reactivity | Human;rat; |
| Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpracticeisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample. |
| Form | Lyophilizedfrom1.2%sodiumacetate,2mgBSA,0.01mgNaN3 |
| Reconstitution | Reconstitutein1mlofPBS(pH7.4)toachieveanantibodyconcentrationof100µg/ml.Centrifugetoremoveanyinsolublematerial. |
| Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
| ExpiryDate | 12monthsafterpurchase |
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