Description | Thetubulinfamilyincludesfivedistincttubulins;alpha,beta,gamma,deltaandepsilon.Gamma-tubulinhasaroleinthenucleationandpolarorientationofmicrotubulesandisfoundatthemicrotubuleorganizingcenterssuchasthespindlepolesorthecentrosome.Theantigensequenceisfoundinbothhumangamma-tubulin1andgamma-tubulin2. |
BatchNo. | Seeproductlabel |
Unitsize | 100µg |
Antigen | Syntheticpeptide(EEFATEGTDRKDVFFY)conjugatedtoKLHcorrespondingtotheregionofaminoacids38-53ofhumangamma-Tubulin. |
Clone | IMD-20 |
OtherNames | Tubulingamma-1chain;Gamma-1-tubulin;Gamma-tubulincomplexcomponent1;GCP-1;TUBG1;TUBG;Tubulingamma-2chain;Gamma-2-tubulin;TUBG2; |
Accession | P23258TBG1_HUMAN;Q9NRH3TBG2_HUMAN; |
Producedin | Mouse |
Purity | IgG |
Applications | WesternBlotting(WB).Aconcentrationof0.25-0.5µg/mlisrecommendedforWB.HumanTubulingamma-1chainhasapredictedlengthof451residuesandMWof51kDa.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWBagainsttheantigen. |
Cross-reactivity | Human;rat; |
Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample. |
Form | Lyophilizedfrom1.2%sodiumacetate,2mgBSA,0.01mgNaN3 |
Reconstitution | Reconstitutein1mlofPBS(pH7.4)toachieveanantibodyconcentrationof100µg/ml.Centrifugetoremoveanyinsolublematerial. |
Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
ExpiryDate | 12monthsafterpurchase |
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