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| Description | Neurofilamentscontainthreeintermediatefilamentproteins:light(68kDa),medium(160kDa)andheavy(200kDa).Neurofilamentheavy(NF200orNF-H)isphosphorylatedanditisthoughtthatthisresultsintheformationofinterfilamentcrossbridgesthatareimportantinthemaintenanceofaxonalcaliber.ThisantibodyrecognisesbothphosphorylatedanddephosphorylatedformsofNF200/NF-H. |
| BatchNo. | Seeproductlabel |
| Unitsize | 100µg |
| Antigen | C-terminalsegmentofenzymaticallydephosphorylatedpigNeurofilamentHeavy(NF200). |
| Clone | NF-200 |
| OtherNames | NF-200;NF200;NF-H;NEFH;N52;Neurofilamentheavypolypeptide;NeurofilamenttripletHprotein;200kDaneurofilamentprotein;KIAA0845;NFH; |
| Accession | P12036NFH_HUMAN;P12037NFH_PIG |
| Producedin | Mouse |
| Purity | IgG |
| Applications | Immunohistochemistry(IHC)andWesternBlotting(WB).Aconcentrationof0.50µg/mlisrecommendedforWB.HumanNF200hasapredictedlengthof1026residuesandMWof112kDa.Aconcentrationof1.0-2.0µg/mlisrecommendedtodetectNF200informalinfixedandparaffinembeddedtissuesaswellasformalin/acetonefixedtissues.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWBandIHCagainsttheantigen. |
| Cross-reactivity | Human;mouse;rat; |
| Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample. |
| Form | Lyophilizedfrom1.2%sodiumacetate,2mgBSA,0.01mgNaN3 |
| Reconstitution | Reconstitutein1mlofPBS(pH7.4)toachieveanantibodyconcentrationof100µg/ml.Centrifugetoremoveanyinsolublematerial. |
| Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
| ExpiryDate | 12monthsafterpurchase. |
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