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| Description | ProteinS100betabelongstotheS100familyofproteinscontaining2EF-handcalciumbindingmotifs.S100proteinsarelocalizedinthecytoplasmand/ornucleusofawiderangeofcells,andareinvolvedintheregulationofanumberofcellularprocessessuchascellcycleprogressionanddifferentiation.Amongbraincells,S100betaisoftenusedasaMarkerforastrocytes.The2subunitsofS100proteinaretheproductsofseparategenes.ThebetasubunitispresentinallS100positivecellsandtumourswhereasthealphasubunitisdetectableonlyinspecifictissuessuchasheart,skeletalmuscleandbrain.TheS100proteinisahomodimercomposedoftwoalphasubunits(S100a)ortwobetasubunits(S100b)orasaheterodimerwithonealphaandbetasubunit(S100aborS100ao).Thisantibodyreactsspecificallywiththebetasubunit. |
| BatchNo. | Seeproductlabel |
| Unitsize | 100µg |
| Antigen | BovinebrainS-100b |
| AntibodyType | Mousemonoclonal |
| Clone | SH-B1 |
| OtherNames | S100B;CalciumbindingproteinB1; |
| Accession | P02638S100B_BOVIN |
| Producedin | Mouse |
| MolecularWeight | ThepredictedlengthofbovineS100betasubunitis92residuesandtheMWofthemonomeris11kDa. |
| Purity | Ascites |
| Applications | Immunohistochemistry(IHC).Aconcentrationof1.0-2.0µg/mLisrecommendedtodetectS100betasubunitinparaffinembeddedtissues.Antigenretrievalbyheatisrecommended.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyIHCagainsttheantigen. |
| SpeciesAgainst | Bovine |
| Cross-reactivity | Human,rabbitandrat.Maycross-reactwithhorseandmonkeyS100-betabasedonaminoacidsequencesimilarities. |
| Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample. |
| Form | Lyophilizedfrommouseascitesfluidcontaining1.2%sodiumacetate,2mgBSA,with0.01mgNaN3aspreservative. |
| Reconstitution | Reconstitutein1mLofPBSbuffertoachieveanantibodyconcentrationof100µg/mL.Centrifugetoremoveanyinsolublematerial. |
| Storage | 12monthsafterpurchaseat-20°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
| ExpiryDate | 12monthsafterpurchase. |
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