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| Description | HeatShockProtein60(HSP60)isachaperoninwithrolesinmitochondrialproteinimportandfoldingofpolypeptidesinthemitochondrialmatrix. |
| BatchNo. | Seeproductlabel |
| Unitsize | 100µg |
| Antigen | Asyntheticpeptide(EYEKEKLNERLAKLSDGV)correspondingtoaregion(384-401)fromhumanHeatShockProtein60(HSP60).Toenhancetheimmunologicalresponse,thispeptidewascoupledtocarrierproteinBSA. |
| OtherNames | Heatshockprotein60;HSP-60;Hsp60;60kDachaperonin;Chaperonin60;CPN60;MitochondrialmatrixproteinP1;P60lymphocyteprotein;HuCHA60;HSPD1;HSP60; |
| Accession | P10809CH60_HUMAN; |
| Producedin | Rabbit |
| Purity | Affinitypurifiedonantigencolumn |
| Applications | Immunohistochemistry(IHC)andWesternBlotting(WB).Aconcentrationof0.5-1.0µg/mlisrecommendedforWB.HumanHSP60hasapredictedlengthof573residuesandMWof61kDa.Aconcentrationof0.5-1.0µg/mlisrecommendedtodetecttheproteininformalinfixedandparaffinembeddedtissues.Antigenretrievalisrequired.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWBandIHCagainsttheantigen. |
| Cross-reactivity | Human;mouse;rat;rabbit; |
| Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample. |
| Form | Liquid(0.5ml).50%glycerol,0.9mgNaCland0.2mgNa2HPO4 |
| Reconstitution | TheliquidformulationshouldbedilutedinPBS(pH7.4) |
| Storage | Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles |
| ExpiryDate | 12monthsafterpurchase |
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