产品资料

EXBIO/Lineage Cocktail FITC/50 tests/6K01-T050

Product details

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Application

FC (QC tested)

Reactivity

Human

Description

The Lineage Cocktail FITC is designed to stain and gate-out human T lymphocytes (CD3⁺), B lymphocytes (CD19⁺CD20⁺), NK cells (CD16⁺CD56⁺), non-differentiated monocytes (CD14⁺), neutrophils (CD16⁺), and eosinophils (CD14^+dim). Peripheral blood dendritic cells and basophils are Lineage negative and do not stain using this reagent. Whenever applicable, the lineage negative cells can be simultaneously stained by other monoclonal antibodies conjugated to appropriate fluorochromes. For example, dendritic cells can be distinguished by positive staining with anti HLA-DR, and basophils by positive staining with anti CD203c.

Contents

Antibody cocktail (1 ml, 50 tests) components: Anti human CD3 (clone MEM-57, IgG2a) FITCAnti human CD14 (clone MEM-15, IgG1)Anti human CD16 (clone LNK16, IgG1)Anti human CD19 (clone LT19, IgG1)Anti human CD20 (clone LT20, IgG2a)Anti human CD56 (clone MEM-188, IgG2a)Stabilizing buffer

Preparation

Premixed combination of mouse monoclonal antibodies labeled with FITC and diluted in optimal concentrations in stabilizing buffer

Formulation

Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide

Storage and handling

Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.

Flow cytometry surface staining pattern of human peripheral whole blood stained using Lineage Cocktail FITC (20 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry multicolor surface staining of human peripheral whole blood stained using Lineage Cocktail FITC (20 μl cocktail / 100 μl of peripheral whole blood) and anti-human CD203c (NP4D6) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human target population cells (red-filled) from basophils (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using Lineage Cocktail FITC (20 μl cocktail / 100 μl of peripheral whole blood).

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EXBIO/Lineage Cocktail FITC/50 tests/6K01-T050