产品资料

EXBIO/Recombinant mouse NTAL / LAB/0.1 mg/EXB0010

Product details

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Application

Reactivity

Mouse

Description

Recombinant mouse NTAL / LAB is expressed in E. coli. DNA sequence encoding mouse NTAL (amino acids 30 - 203) was fused with a His tag at the N-terminus.A calculated molecular mass of the fussion protein is 22.2 kDa. The recombinant fragment migrates as a 28 kDa protein in SDS-PAGE under reducing conditions.

Contents

Recombinant mouse NTAL / LAB - 1 vial (0.1 mg). Reconstitute vial content with 100 μl sterile deionized water. For long term storage or for preparation of more diluted working stock solution, a carrier protein (e.g. 1% serum albumin) should be added to the vial.

Preparation

The protein was purified by immobilized metal affinity chromatography used for purification of polyhistidine-tagged recombinant proteins, and lyophilized.

Formulation

Lyophilized from a 0.22 μm filtered solution in phosphate buffered saline (PBS).

Storage and handling

Store lyophilized product in freezer (-20 °C to -80 °C). The expiration date of unopened product vial is indicated on the vial label. After reconstitution prepare suitable aliquots. Reconstituted product is stable for at least 6 months when stored in freezer (-20 °C to -80 °C). Avoid freeze/thaw cycles.

Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.

Western Blotting analysis of mouse NTAL / LAB recombinant protein (Cat. No. EXB0010, previously RL-005-C100). SDS-PAGE Electrophoresis (12% separating gel, 6% stacking gel; reducing conditions).Lane 1 - 4: immunostaining with NTAL specific antibody; dilution 2 μg/ml). Detection with Goat anti-mouse/ HRP secondary antibody. Amount of mouse NTAL / LAB recombinant protein loaded on each lane: (1) 50 ng, (2) 20 ng, (3) 5 ng, (4) 1 ng.

SDS-PAGE Electrophoresis of mouse NTAL / LAB recombinant protein (Cat. No. EXB0010, previously RL-005-C100; 12% separating gel, 6% stacking gel; reducing conditions).Lane 1 - 4: mouse NTAL / LAB recombinant protein (2000 ng, 1000 ng, 500 ng, 200 ng per lane). Staining with Coomassie Blue.

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EXBIO/Recombinant mouse NTAL / LAB/0.1 mg/EXB0010