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EXBIO/Recombinant human Cbp / PAG/0.1 mg/EXB0009

Product details

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References

Application

Reactivity

Human

Description

Recombinant human PAG / Cbp is expressed in E. coli.DNA sequence encoding intracellular domain of human Cbp/PAG (amino acids 41- 432) was fused with a His tag at the N-terminus.A calculated molecular mass of fussion protein is 45.3 kDa. As a result of highly acidic character of Cbp/PAG (predicted isoelectric point pI 4.4) and further modification by phosphorylation, the recombinant protein migrates as a 70 - 85 kDa protein in SDS-PAGE under reducing conditions.

Contents

Recombinant human PAG / Cbp - 1 vial (0.1 mg).Reconstitute vial content with 100 μl sterile deionized water. For long term storage or for preparation of more diluted working stock solution, a carrier protein (e.g. 1% serum albumin) should be added to the vial.

Preparation

The protein was purified by immobilized metal affinity chromatography used for purification of polyhistidine-tagged recombinant proteins, and lyophilized.

Formulation

Lyophilized from a 0.22 μm filtered solution in phosphate buffered saline (PBS).

Storage and handling

Store lyophilized product in freezer (-20 °C to -80 °C). The expiration date of unopened product vial is indicated on the vial label. After reconstitution prepare suitable aliquots. Reconstituted product is stable for at least 6 months when stored in freezer (-20 °C to -80 °C). Avoid freeze/thaw cycles.

Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.

SDS-PAGE Electrophoresis of Cbp / PAG recombinant protein (cat. no. EXB0009, previously RL-004-C100). 12% separating gel, 6% stacking gel; reducing conditions.Lane 1-5: Cbp / PAG recombinant protein (2000 ng, 1000 ng, 500 ng, 200 ng, 100 ng per lane). Staining with Coomassie Blue.

Western Blotting analysis of Cbp / PAG recombinant protein (cat. no. EXB0009, previously RL-004-C100). Reducing conditions.Lane 1-4: immunostaining with anti-Cbp rabbit polyclonal antibody (EXBIO cat. no. 11-409-C100; dilution 0.5 μg/ml). Detection with Goat anti-rabbit / HRP secondary antibody. Amount of Cbp/PAG recombinant protein loaded on each lane: (1) 100 ng, (2) 50 ng, (3) 20 ng, (4) 10 ng.

Product specific references:

Brdicka T, Pavlistova D, Leo A, Bruyns E, Korinek V, Angelisova P, Scherer J, Shevchenko A, Hilgert I, Cerny J, Drbal K, Kuramitsu Y, Kornacker B, Horejsi V, Schraven B. Phosphoprotein associated with glycosphingolipid-enriched microdomains (PAG), a novel ubiquitously expressed transmembrane adaptor protein, binds the protein tyrosine kinase csk and is involved in regulation of T cell activation. J Exp Med. 2000 May 1;191(9):1591-604.PubMed

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EXBIO/Recombinant human Cbp / PAG/0.1 mg/EXB0009

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