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TheImmunoStar5-HT2Creceptorantiserumwasqualitycontroltestedusingstandardimmunohistochemicalmethods.Theantiserumdemonstratesstronglypositivelabelingofratchoroidplexusandhippocampususingindirectimmunofluorescentandbiotin/avidin-HRPtechniques.Recommendedprimarydilutionsare/500-1/1000inPBS-Bn/Av-HRPtechnique.Intensificationmethodssuchasnickelwillapproximatelydoublethedilutionfactorasrecommended.
TheantibodywascharacterizedbyimmunohistochemistryandWesternblot.Westernblottingrevealedasinglebandofapproximately70kD.
Preincubationoftheantibodywithanexcessofthesyntheticpeptideblockedstaining.ImmunohistochemicalstainingofratbraincorrelateswellwithNorthernanalysis,insituhybridizationandreceptorautorADIography.BlastPdatabasesequencehomologysearchesconfirmedthatthissequenceisuniquetorat,mouseandhuman5-HT2Creceptors.
PhotoDescription:GrayscaleIHCimageofcoronalratbrainsectionstainingforthe5-HT2Creceptor(above)andhighmagnificationimageofreceptorexpressioninthecortexoftheratbrain(below).Thetissuewasfixedwith4%formaldehyde/0.05%glutaraldehydein0.1Mphosphatebuffer,beforebeingremovedandpreparedforvibratomesectioning.FloatingsectionswereincubatedatRTin10%goatseruminPBS,beforestandardIHCprocedure.Primaryantibodywasincubatedat1:1000for48hours,goatanti-rabbitsecondarywassubsequentlyaddedfor1hourafterwashingwithPBS.Lightmicroscopystainingwasachievedwithstandardbiotin-streptavidin/HRPprocedureandDABchromogen.
Host: Rabbit
Quantity/Volume: 100µL
State: Liquid
ReactsWith:Mouse,Rat
AlternateNames: 5-hydroxytryptaminereceptor2C;5-HT2C;5HT-1C;5-HTR2C;5-hydroxytryptamine(SEROtonin)receptor2C,Gprotein-coupled,anti-5-HT2C
RRID:AB_572212
Immunogen:Rat5-HT2Creceptor(439-460)
GeneSymbol/ID,Accession#: Htr2c,25187
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vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
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GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn