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TheImmunoStar5-HT5AReceptorwasqualitycontroltestedusingstandardimmunohisto-chemicalmethods.Theantiserumdemonstratesstronglypositivelabelingofratcortexandhippocampususingindirectimmunofluorescentandbiotin/avidin-HRPtechniques.Recommendedprimarydilutionis1/200-1/400inPBS/0.3%TritonX-100-Bn/Av-HRP.Intensificationmethodssuchasnickelwillapproximatelydoublethedilutionfactorasrecommended.
Theantibodywascharacterizedbyimmunoblottingandimmunohistochemistry.Immunoblotsofratbrainextractsrevealedthepresenceoftwobandsatmolecularweightsof41and47kD.Thelowerweightbandagreeswiththecalculatedmolecularweightbasedonaminoacidsequence.Thehigherweightmayrepresentglycosylatedreceptorprotein.
ImmunohistochemicalstainingofratbraincorrelateswellwithNorthernblotanalysisandinsituhybridizationstudies.Immunolabelingiscompletelyabolishedbypreadsorptionwithsyntheticrat5-HT5Areceptor(17-34).BlastPdatabasesequencehomologysearchesindicatethattheaminoacidsequenceisuniquetoratandmouse5-HT5Areceptor.
PhotoDescription:IHCimageofneuronsstainingforthe5-HT5Areceptorintherathippocampus(above)andtheratcortex(below).Thetissuewasfixedwith4%formaldehyde/0.05%glutaraldehydein0.1Mphosphatebuffer,beforebeingremovedandpreparedforvibratomesectioning.FloatingsectionswereincubatedatRTin10%goatseruminPBS,beforestandardIHCprocedure.Primaryantibodywasincubatedat1:500for48hours,goatanti-rabbitsecondarywassubsequentlyaddedfor1hourafterwashingwithPBS.Lightmicroscopystainingwasachievedwithstandardbiotin-streptavidin/HRPprocedureandDABchromogen.
Host: Rabbit
Quantity/Volume: 100µL
State: Liquid
ReactsWith:Clam,Rat
AvailABIlity:InStock
AlternateNames: 5-hydroxytryptaminereceptor5A;5-HT5A;REC17;5ht5a,MR22;5-hydroxytryptamine(SEROtonin)receptor5A,Gprotein-coupled,anti-5-HT5a
RRID:AB_572213
Immunogen:Rat5-HT5Areceptor(17-34)
GeneSymbol/ID,Accession#: Htr5a,25689
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