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Theantibodyproducesmoderatelabelingofrapheneuronsinnormalrat.InratswhoseSEROtonergicsystemhasbeenactivated,stainingintensityisincreasedtoamaximumlabel.Recommendeddilutionoftheantiserumis1/4000-1/8000forbiotin-streptavidin/HRPtechnique.
Thespecificityoftheantiserumwasevaluatedusingamodelsystemofgelatin-indoleplugsbyamethodsimilartopublishedprocedures(SchipperandTilders,1983).Resultsshowedthatthe5-HIAAantibodydosedependentlystained5-HIAAbutdidnotstainanyconcentrationof5-HTor5-HTP.Theantiserumwasalsotestedbypre-adsorptionat25µg/mLwithvariousBSAconjugates.Whilepre-adsorptionwith5-HIAAconjugatecompletelyeliminatesimmunolabeling,pre-adsorptionwithconjugatesof5-HT,5-HTPanddopaminehadnoeffectonstainingintensityordistributionofstain.
PhotoDescription:IHCimageofneuronsstainingfor5-H1AAintheraphenucleusoftheratbrainstem.Thetissuewasfixedwith4%formaldehydeinphosphatebuffer,beforebeingremovedandpreparedforvibratomesectioning.FloatingsectionswereincubatedatRTin10%goatseruminPBS,beforestandardIHCprocedure.Primaryantibodywasincubatedat1:5000for48hours,goatanti-rabbitsecondarywassubsequentlyaddedfor1hourafterwashingwithPBS.Lightmicroscopystainingwasachievedwithstandardbiotin-streptavidin/HRPprocedureandDABchromogen.
Host:Rabbit
Quantity/Volume:100µL
State:LyophilizedWholeSerum
ReactsWith::Rat,TritoniaDiomedea(SeaSlug)
AlternateNames:anti-5-HIAA
RRID:AB_572208
Immunogen:5-HIAA
GeneSymbol:SLC6A4
EntrezGeneID:6532
NCBIGeneAliases:5-HTT,5-HTTLPR,5HTT,HTT,OCD1,SERT,hSER
matriks/Shikari® (S-ATP) Anti-Pembrolizumab ELISA w/confirmation/PEM-QNS-KEY
intactgenomics/Methionine Auxotrophic LBA4404 | ig® | Intact Genomics/15x50μl/1076-15
goprolytix/Proteolytic Activation of Prothrombin/1 mg, 200 µg/BCT-DFP
novateinbio/Dynorphin A ( 13-17 ) Porcine Peptide/5mg/PE-54024_5mg
matriks/Shikari® (Q-ETA) Etanercept ELISA/ETA-FD-ENB
intactgenomics/Agrobacterium ElectroComponent Combo Pack | Intact Genomics/12x50µl/1290-24
Jackson/Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L)/2.0 ml/115-035-003
Jackson/Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L)/2.0 ml/111-035-003
Qubit™ dsDNA BR Assay Kit Q32853现货
Invitrogen Q32855 Qubit™ RNA HS Assay Kit现货促销
LUCK-1G,D-Luciferin, Potassium Salt 钾盐现货
GoldBio/D-Luciferin, Sodium Salt (Proven and Published™)/LUCNA-1G/1 g