Theantibodyproducesmoderatelabelingofrapheneuronsinnormalrat.InratswhoseSEROtonergicsystemhasbeenactivated,stainingintensityisincreasedtoamaximumlabel.Recommendeddilutionoftheantiserumis1/4000-1/8000forbiotin-streptavidin/HRPtechnique.
Thespecificityoftheantiserumwasevaluatedusingamodelsystemofgelatin-indoleplugsbyamethodsimilartopublishedprocedures(SchipperandTilders,1983).Resultsshowedthatthe5-HIAAantibodydosedependentlystained5-HIAAbutdidnotstainanyconcentrationof5-HTor5-HTP.Theantiserumwasalsotestedbypre-adsorptionat25µg/mLwithvariousBSAconjugates.Whilepre-adsorptionwith5-HIAAconjugatecompletelyeliminatesimmunolabeling,pre-adsorptionwithconjugatesof5-HT,5-HTPanddopaminehadnoeffectonstainingintensityordistributionofstain.
PhotoDescription:IHCimageofneuronsstainingfor5-H1AAintheraphenucleusoftheratbrainstem.Thetissuewasfixedwith4%formaldehydeinphosphatebuffer,beforebeingremovedandpreparedforvibratomesectioning.FloatingsectionswereincubatedatRTin10%goatseruminPBS,beforestandardIHCprocedure.Primaryantibodywasincubatedat1:5000for48hours,goatanti-rabbitsecondarywassubsequentlyaddedfor1hourafterwashingwithPBS.Lightmicroscopystainingwasachievedwithstandardbiotin-streptavidin/HRPprocedureandDABchromogen.
Host:Rabbit
Quantity/Volume:100µL
State:LyophilizedWholeSerum
ReactsWith::Rat,TritoniaDiomedea(SeaSlug)
AlternateNames:anti-5-HIAA
RRID:AB_572208
Immunogen:5-HIAA
GeneSymbol:SLC6A4
EntrezGeneID:6532
NCBIGeneAliases:5-HTT,5-HTTLPR,5HTT,HTT,OCD1,SERT,hSER
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn