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ImmunoStar/5-HT (Serotonin) Goat Antibody/20079/1 Ea188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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ImmunoStar/5-HT (Serotonin) Goat Antibody/20079/1 Ea

Description

TheImmunoStarSEROtoninantiserumwasqualitycontroltestedusingstandardimmunohistochemicalmethods.Theantiserumdemonstratesstronglypositivelabelingofrathypothalamusandspinalcordusingindirectimmunofluorescentandbiotin/avidin-HRPtechniques.Recommendedprimarydilutionis1/5000-1/10,000inPBS/0.3%TritonX-100-Bn/Av-HRPTechnique.

Stainingiscompletelyeliminatedbypretreatmentofthedilutedantibodywith100µgofserotonin/BSAconjugate. 

PhotoDescription:LowmagnificationofIHCimageofneuronsstainingforthe5-HTgoatantibodyinthepontineregionoftheratbrain(top),andhighmagnificationoftheraphenucleus(bottom).Thetissuewasfixedwith4%formaldehyde/0.05%glutaraldehydein0.1Mphosphatebuffer,beforebeingremovedandpreparedforvibratomesectioning.FloatingsectionswereincubatedatRTin10%rabbitseruminPBS,beforestandardIHCprocedure.Primaryantibodywasincubatedat1:7500for48hours,rabbitanti-goatsecondarywassubsequentlyaddedfor1hourafterwashingwithPBS.Lightmicroscopystainingwasachievedwithstandardbiotin-streptavidin/HRPprocedureandDABchromogen.

5HT Goat raphe nucleus #20079

Host:Goat

Quantity/Volume:100µL

State:LyophilizedWholeSerum

AvailABIlity:InStock

ReactsWith:Cat,Cod,Cow,Crayfish,Dolphin,Fish,Frog(XenopusLaevis),GuineaPig,Hamster,Human,Mexicansalamander(Ambystoma Mexicanum),Monkey,Moth,Mouse,Pig,Rabbit,Ram(Sheep),Rat,Salamander,Snail,Tapeworm,Worm,Zebrafish

AlternateNames: anti-5-HTGoat

RRID: AB_572262

Immunogen:Serotonin

GeneSymbol:Spl

GeneID:104153

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