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Innova Biosciences/Latex conjugation kit – 400nm Black/10040100/10 reaction mini kit

Our Latex Conjugation Kit allows antibodies or proteins to be conjugated to our high quality 400nm black latex beads quickly and easily. The latex beads are designed for ease of use and have much improved handling compared with traditional latex. This has been achieved by specially treating the particles. The conjugation is covalent, and requires less antibody than traditional passive and covalent latex conjugation.

The 400 nm black latex beads in this kit are freeze dried. The conjugation reaction is initiated simply by reconstituting the dry mixture with your antibody, which becomes attached (via lysine residues) to the specially treated surface.

LATEX conjugation procedure

  • 30 seconds to set up the conjugation
  • 3 minutes hands-on time for the conjugation procedure
  • Conjugate is ready to use within 35 minutes. You simply pipette the biomolecule into a vial containing the latex nanoparticles, then centrifuge to buffer exchange.

Please refer to our custom services for conjugation services or get in contact with us to know more about this product.

Figure 1. Lateral Flow Assay of our specially-treated latex beads conjugated to a monoclonal anti-CRP antibody (mAb1) titrated against CRP. 2 ng/ml CRP can easily be detected.

Figure 2. Lateral Flow Assay of our specially-treated latex conjugated to the monoclonal anti-CRP antibody (mAb 2) titrated against CRP in buffer or CRP spiked CRP-depleted serum (100 % serum). The latex behaves similarly in serum and buffer, and no aggregation or non-specific binding is seen.

Figure 3. Multiplexed Latex. The three colours of latex each conjugated to a different antibody or protein and forming a line either by a direct binding event (red and black latex) or a sandwich assay binding event (blue latex). The three colours of latex demonstrate no aggregation or background staining.

Towards a point-of-care strip test to diagnose sickle cell anemia - PLoS One (2017)

View reference

Two-Color Lateral Flow Assay for Multiplex Detection of Causative Agents Behind Acute Febrile Illnesses - Analytical Chemistry (2016)

View reference


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