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Innova Biosciences/Lightning-Link® APC/Cy5.5/764-0015/1 reaction up to 1mg

TheInnovaBiosciencesLightning-Link®APC/Cy5.5antibodylabelingkitenablesthedirectconjugationofantibodies,proteinsandpeptides,oranyotherbiomoleculewithanavailableaminegroup,toAPC/Cy5.5withonly30secondshands-ontimeandwith100%recoveryofmaterials.Thetechnologyisfullyscalablefrom10ugto1gormoreandstringentlyQCtestedforconsistenthighqualityandexcellentbatch-to-batchreproducibility.

Lightning-Link_process_diagram

APC/Cy5.5isatandemconjugate.TheAPCisexcitedat652nmandfunctionsasanenergydonorfortheCy5.5.EnergyistransferredfromtheAPCtotheCy5.5viaFRET(FluorescenceResonanceEnergyTransfer).TheCy5.5emitsthentheenergyreceivedfromtheAPCintheformoflongwavelengthlightat700nm.

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FlowCytometrydata–APC/Cy5.5

Mouseanti-humanCD3wasconjugatedwithAPC/Cy5.5usinganInnovaBiosciencesLightning-Link®kit.Theconjugatedantibodywasthenusedtostainhumanperipheralbloodlymphocytes,followedbyanalysiswithflowcytometry.(Blueline–negativecontrol;redline–positivestaining)

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