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Innova Biosciences/High Throughput Colorimetric ATPase Assays (inc. PiColorLock)/601-0121/480 assays

ThisnonrADIoactivecolorimetricassaykitcontainsallthenecessaryreagentsformeasuringenzymeactivity(everythingincludedinthePiColorlock™reagentandmore)andisidealforhighthroughputdrugscreening.

ThekitscontainPiColorlock™,anon-radioactive,superiorphosphatedetectionreagent.

KeyfeaturesofPiColorLock™include:

  • ColorimetricAssay–competitorassaysareradioactive
  • Specialadditiveensureslowbackgroundswithacid-lABIlesubstrates
  • Unparalleledstabilityofphosphate-dyecomplexes
  • ReagentiscompatIBLewithalmostanyassaybuffer
  • Noinhibitionofcolordevelopmentbyhighconcentrationsofprotein
  • Stablereagentformulation–longshelflife

ColorimetricassaysforATPasesareinvariablybasedontheformationofcoloredcomplexesbetweenaninorganicphosphateandadyemoleculeunderacidicconditions.

Comparisonwithcompetitors:

Competitorassaysarebesetwithseveralproblemsincludingreagentprecipitation.ThehighstabilityofPiColorLock™ensureshighstabilityofthecoloreddye-phosphatecomplexes(greencolor-seebelow).

 


Figure1.ATPhasbeenincubatedinthreedetectionreagents.Asteadilyrisingbackgroundsignalisseenwithcompetitorreagents,whereasPiColorLock™givesbaselinereadings.

Figure2.PiColorLockTMhasalsobeendesignedtohavealargelinearrange,thusreducingtheneedforsampledilution.Competitors’productsarelinearoveramuchnarrowerrangeofconcentrations.

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