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Innova Biosciences/Biotin Check&Go!/4001-0030/30 strips

BiotinCheck&Go!allowsscientiststoconfirmthesuccessoftheirantibodybiotinylationinoneeasystep.

UnliketrADItionalmethodsofassessingthesuccessofbiotinylation,suchastheHABAassay,whichcanbetediousandtimeconsuming,BiotinCheck&Go!isasimpleimmunochromatographytestthatrequiresonly10minutesofyourtimeandverysmallquantitiesofyourantibodyconjugate.

ThekeycomponentsofthekitincludeanitrocellulosemembranecontainingaTestlineofimmobilizedProteinAandProteinG,bothwhichhaveahighaffinityfortheFcregionofavarietyofIgGmolecules,anda40nmInnovaCoat®GOLDStreptavidinconjugatefordetection.

  • Quicklycheckthesuccessofyourconjugation(only10minutes!)
  • Easytouse
  • Nocostlyequipmentrequired
  • CompatIBLewithantibodyconjugatesgeneratedusingourLightning-Link®Biotinantibodylabelingkitsandotherbiotinlabelingtechnologiesavailablecommercially
  • Requiresonlysmallvolumes(20ulofdilutedconjugate)
  • UniquetoInnovaBiosciences

Whenthebiotinylatedantibodyismixedwiththestreptavidin-goldconjugateitformsacomplexthatflowsalongthenitrocellulosemembrane.UponbindingtotheProteinAandProteinGconcentratedontheTestline,avisibleredlineappearsonthestrip.

 

Figure1.RabbitIgGhasbeenconjugatedwithLightning-Link®RapidBiotinAandrunonProteinA/Gstripsatdifferentconcentrationstogetherwith0.4ODInnovaCoat®GOLDStreptavidin.TheintensityoftheT-lineisproportionaltotheconcentrationofthebiotinylatedIgGs.AredlineappearsevenatlowIgGsconcentration.

 

Figure2.Polyclonalantibodiesfromrabbit,goat,mouseandrathavebeenconjugatedwithLightning-Link®RapidBiotinAandrunonProteinA/Gstripsatdifferentconcentrationstogetherwith0.4ODInnovaCoat®GOLDStreptavidin.Antibodiesfromallspeciesprovidedaredvisiblelineonthestrips.

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