The Innova Biosciences Lightning-Link® B-Phycoerythrin (BPE) antibody labeling kit enables the direct conjugation of antibodies, proteins and peptides, or any other biomolecule with an available amine group, to B-Phycoerythrin (BPE) with only 30 seconds hands-on time and with 100% recovery of materials. The technology is fully scalable from 10ug to 1g or more and stringently QC tested for consistent high quality and excellent batch-to-batch reproducibility.
BPE is a fluorescent protein from the phycobiliprotein family from the cyanbacteria and eukaryotic algae. It has a strong absorption peak at about 546 nm and has an emission value of 580 nm.
B-Phycoerythrin is often thought to be less “sticky” than R-Phycoerythrin therefore BPE conjugates tend to produce less background signal from non-specific binding in certain applications.
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Figure 1. Mouse anti-human CD3 was conjugated with B Phycoerythrin using an Innova Biosciences Lightning-Link® kit. The conjugated antibody was then used to stain human peripheral blood lymphocytes, followed by analysis with flow cytometry. (Blue line – negative control; red line – positive staining).