Innova Biosciences/High Throughput Colorimetric ATPase Assays (inc. PiColorLock)/6010120/192 assays

This non radioactive colorimetric assay kit contains all the necessary reagents for measuring enzyme activity (everything included in the PiColorlock™ reagent and more) and is ideal for high throughput drug screening.

The kits contain PiColorlock™, a non-radioactive, superior phosphate detection reagent.

Key features of PiColorLock™ include:

Colorimetric Assay – competitor assays are radioactive
Special additive ensures low backgrounds with acid-labile substrates
Unparalleled stability of phosphate-dye complexes
Reagent is compatible with almost any assay buffer
No inhibition of color development by high concentrations of protein
Stable reagent formulation – long shelf life

Colorimetric assays for ATPases are invariably based on the formation of colored complexes between an inorganic phosphate and a dye molecule under acidic conditions.

Comparison with competitors:

Competitor assays are beset with several problems including reagent precipitation. The high stability of PiColorLock™ ensures high stability of the colored dye-phosphate complexes (green color- see below).

Figure 1. ATP has been incubated in three detection reagents. A steadily rising background signal is seen with competitor reagents, whereas PiColorLock™ gives baseline readings.

Figure 2. PiColorLock^TM has also been designed to have a large linear range, thus reducing the need for sample dilution. Competitors’ products are linear over a much narrower range of concentrations.

对照品标准品，生物试剂盒，gibco胎牛血清，试剂采购网，中国试剂网，细胞因子试剂盒

Innova Biosciences/High Throughput Colorimetric ATPase Assays (inc. PiColorLock)/6010120/192 assays

Key features of PiColorLock™ include:

Comparison with competitors:

Protein tyrosine phosphatase 1B is a mediator of cyclic ADP ribose-induced Ca2+ signaling in ventricular myocytes - Exp Mol Med. (2017)

Piglets Are Vulnerable to Intestinal Inflammation and Infection Because of Expression of Low-Affinity Jejunal Alkaline Phosphatase Activities - The FASEB Journal (2017)

Structural studies of apolipoprotein A-I and ATP-binding cassette A1 and their roles in nascent high density lipoprotein biogenesis - Boston University Theses & Dissertations (2017)

Dissecting biochemical peculiarities of the ATPase activity of TcSub2, a component of the mRNA export pathway in Trypanosoma cruzi - Int J Biol Macromol. (2017)

Identification and Characterization of Novel Compounds Blocking Shiga Toxin Expression in Escherichia coli O157:H7 - Front. Microbiol. (2016)

The Biophysical Characterisation and SAXS Analysis of Human NLRP1 Uncover a New Level of Complexity of NLR Proteins - PLoS One (2016)

ARD1-mediated Hsp70 acetylation balances stress-induced protein refolding and degradation - Nature Commun. (2016)

Isolation of Intact and Functional Melanosomes from the Retinal Pigment Epithelium - PLoS One (2016)

Characterization of a cadmium resistance Lactococcus lactissubsp. lactis strain by antioxidant assays and proteome profiles methods - Environmental Toxicology and Pharmacology (2016)

Dual ATPase and GTPase activity of the replication-associated protein (Rep) of beak and feather disease virus - Virus Res. (2015)

Structure-based mutagenesis of STIV B204 reveals essential residues in the virion-associated DNA packaging ATPase - J. Virol. (2016)

Functional Characterization of ABCA3 Mutations from Infants with Respiratory Distress Syndrome - Am. J. Respir. Cell Mol. Biol. (2016)

Remote ischemic perconditioning attenuates ischemia/reperfusion?induced downregulation of AQP2 in rat kidney - Physiol. Rep. (2016)

Antifungal Activity and Biochemical Response of Cuminic Acid against Phytophthora capsici Leonian - Molecules (2016)

Proteome Profiling in the Rat Harderian Gland - J Proteome Res (2006)

Identification, structure and mode of action of a new regulator of the Helicobacter pylori HP0525 ATPase - EMBO J. (2007)

First identification and functional characterization of an immunogenic protein in unculturable haemotrophic Mycoplasmas (Mycoplasma suis HspA1) - FEMS Immunology & Medical Microbiology (2007)

Consumption of Soy Protein Isolate Modulates the Phosphorylation Status of Hepatic ATPase/ATP Synthase ß Protein and Increases ATPase Activity in Rats - J. Nutr. (2007)

Hippocampal levels of gamma-enolase, C-1-tetrahydrofolate synthase and serotransferrin fluctuate over the estrous cycle in the rat - Neuroscience (2008)

Functional characterization of the nucleotide binding domain of the Cryptosporidium parvum CpABC4 transporter: An iron–sulfur cluster transporter homolog - Molecular and Biochemical Parasitology (2008)

The Two-component NS2B-NS3 Proteinase Represses DNA Unwinding Activity of the West Nile Virus NS3 Helicase - JBC (2008)

ATP scavenging by the intracellular pathogen Porphyromonas gingivalis inhibits P2X7-mediated host-cell apoptosis - Cell Microbiol. (2008)

Staphylococcus aureus HrtA Is an ATPase Required for Protection against Heme Toxicity and Prevention of a Transcriptional Heme Stress Response - Journal of Bacteriology (2008)

An Afg2/Spaf-Related Cdc48-like AAA ATPase Regulates the Stability and Activity of the C. elegans Aurora B Kinase AIR-2 - Developmental Cell (2008)

Stress-Dependent Coordination of Transcriptome and Translatome in Yeast - PLoS Biology (2009)

ATP and MO25? Regulate the Conformational State of the STRAD? Pseudokinase and Activation of the LKB1 Tumour Suppressor - PLoS Biology (2009)

Evidence That Two ATP-Dependent (Lon) Proteases in Borrelia burgdorferi Serve Different Functions - PLoS Pathogens (2009)

NS4A regulates the ATPase activity of the NS3 helicase: a novel cofactor role of the non-structural protein NS4A from West Nile virus - Journal of General Virology (2009)

Apratoxin A Reversibly Inhibits the Secretory Pathway by Preventing Cotranslational Translocation - Molecular Pharmacology (2009)

ATP and MO25? Regulate the Conformational State of the STRAD? Pseudokinase and Activation of the LKB1 Tumour Suppressor – PLoS Biol (2009)

Crystal Structure of a Novel Conformational State of the Flavivirus NS3 Protein: Implications for Polyprotein Processing and Viral Replication - J Virol (2009)

Stress-Dependent Coordination of Transcriptome and Translatome in Yeast - PLoS Biology (2009)

Biosynthesis of (Bacterio)chlorophylls ATP-Dependent Transient Subunit Interaction And Electron Transfer Of Dark Operative Protochlorophyllide Oxireductase - JBC (2010)

Modulation of a Schistosoma mansoni multidrug transporter by the antischistosomal drug praziquantel - The FASEB Journal (2010)

Lipopeptide induces apoptosis in fungal cells by a mitochondria-dependent pathway - Peptides (2010)

Arabidopsis stromal 70-kDa heat shock proteins are essential for chloroplast development - Planta (2010)

ATP-binding cassette-like transporters are involved in the transport of lignin precursors across plasma and vacuolar membranes - PNAS (2010)

Identification of gp96 as a Novel Target for Treatment of Autoimmune Disease in Mice – PLoS One (2010)

Biochemical Dissection of the ATPase TraB, the VirB4 Homologue of theEscherichia coli pKM101 Conjugation Machinery - J. Bacteriol. (2010)

Monoclonal antibodies against nucleoside triphosphate hydrolase-II can reduce the replication of Toxoplasma gondii - Parasitology (2010)

Modulation of UvrD Helicase Activity by Covalent DNA-Protein Cross-links - Biol Chem. (2010)

Antimicrobial Mechanism of Action of Transferrins: Selective Inhibition of H+-ATPase - Antimicrob Agents Chemother. (2010)

Identification of gp96 as a Novel Target for Treatment of Autoimmune Disease in Mice - PLoS One (2010)

Overexpression of the Endoplasmic Reticulum Chaperone BiP3 Regulates XA21-Mediated Innate Immunity in Rice – PLoS One (2010)

A Common Variant in TFB1M Is Associated with Reduced Insulin Secretion and Increased Future Risk of Type 2 Diabetes - Cell Metabolism (2011)

Functional defect caused by the 4544G>A SNP in ABCC2: potential impact for drug cellular disposition - Pharmacogenetics and Genomics (2011)

Digitoxin and a synthetic monosaccharide analog inhibit cell viability in lung cancer cells - Toxicology and Applied Pharmacology (2011)

Enzyme Stability of Microencapsulated Bifidobacterium animalis ssp. lactis Bb12 after Freeze Drying and during Storage in Low Water Activity at Room Temperature - Journal of Food Science (2011)

hSWS1·SWSAP1 Is an Evolutionarily Conserved Complex Required for Efficient Homologous Recombination Repair - JBC (2011)

Xeroderma pigmentosum group F protein binds to Eg5 and is required for proper mitosis: implications for XP-F and XFE - Genes to Cells (2012)

Peroxisomal proteostasis involves a Lon family protein that functions as protease and chaperone - JBC (2012)

Structure of the VirB4 ATPase, alone and bound to the core complex of a type IV secretion system - PNAS (2012)

Common Pathogenic Effects of Missense Mutations in the P-Type ATPase ATP13A2 (PARK9) Associated with Early-Onset Parkinsonism - PLOS One (2012)

Caveolin-1 regulates proliferation and osteogenic differentiation of human mesenchymal stem cells - Journal of Cellular Biochemistry (2012)

Novel 2, 4-Pyrimidine Derivatives And Use Thereof - European Patent (2012)

P. gingivalis-Nucleoside-diphosphate-kinase Inhibits ATP-Induced Reactive-Oxygen-Species via P2X7 Receptor / NADPH-Oxidase Signaling and Contributes to Persistence - Cellular Microbiology (2012)

Mutations in DNA2 Link Progressive Myopathy to Mitochondrial DNA Instability - American Journal of Human Genetics (2013)

Survival of Bifidobacterium longum 1941 microencapsulated with proteins and sugars after freezing and freeze drying - Food Research International (2013)

Bcl-2 associated athanogene 5 (Bag5) is overexpressed in prostate cancer andinhibits ER-stress induced apoptosis - BMC Cancer (2013)

Potent in vitro Interference of Fleroxacin in DNA-binding, Unwinding andATPase Activities of Bloom Helicase - Biomedical and Environmental Sciences (2013)

Antagonistic Interaction between Adenosine A2A Receptors and Na+/K+-ATPase-?2 Controlling Glutamate Uptake in Astrocytes - Journal of Neuroscience (2013)

Deregulation of Mitochondrial ATPsyn-? in Acute Myeloid Leukemia Cells and with Increased Drug Resistance - PLoS One (2013)

Structure of CbpA J-Domain Bound to the Regulatory Protein CbpM Explains Its Specificity and Suggests Evolutionary Link between CbpM and Transcriptional Regulators - PLoS One (2014)

A helminth cathelicidin-like protein suppresses antigen processing and presentation in macrophages via inhibition of lysosomal vATPase - The FASEB Journal (2014)

Suramin Inhibits Hsp104 ATPase and Disaggregase Activity - PLoS One (2014)

Physiological and biochemical characteristics of laboratory induced mutants of Botrytis cinerea with resistance to fluazinam - Pesticide Biochemistry and Physiology (2014)

Cyclic nucleotide dependent ATPase activity in inside out vesicles from human erythrocytes - Masters Thesis (2015)

Double-stranded DNA translocase activity of transcription factor TFIIH and the mechanism of RNA polymerase II open complex formation - PNAS (2015)

Herpesviral G Protein-Coupled Receptors Activate NFAT to Induce Tumor Formation via Inhibiting the SERCA Calcium ATPase - PLoS Pathogens (2015)

The MinD homolog FlhG regulates the synthesis of the single polar flagellum of Vibrio alginolyticus - Molecular Microbiology (2015)