产品资料

LifeSensors/DU0101-K48 PANEL/DU0101-K48 PANEL

IQFDiUbTechnology

LifeSensors’sdiubiquitinsubstratesrepresentanewclassofsubstratesforthecontinuousfluorescentmeasurementoftrueisopeptidaseactivity.TheC-terminusofwildtypeubiquitinisconjugatedviaanisopeptidebondtolysine48(K48)ofasecondubiquitinmoleculewiththeresultantdiubiquitinforminganinternallyquenchedfluorescentFRETpair(IQF).Eachubiquitinislabeledwithasinglemoleculeofeitherafluorescentreporter(i.e.TAMRA)orahighlyefficientquenchingfluorophore.CleavageoftheIQFDiUbbydeubiquitylasesleadstoseparationofthefluorophorefromquencherandsubsequentincreaseinobservedfluorescence.

IQFDiUbVariants

ThereisaremarkabledifferencebetweenK48-andK63-linkedubiquitinmoleculesintheirthree-dimensionalstructure,whichaccountsfortheirindividualfunctions.Beyonddifferentiallinkages(e.g.K48,K63,K11),LifeSensorshascreatedsubpanelsofIQFdiubiquitinsubstrateswithineachlinkage.BecauseeachDUBislikelytorecognizeandcleavesubstrateswithuniquestericconsiderations,thesesubpanelsvaryinlocationofreporterfluorophoreandquencher.ItisrecommendedthateachDUBbeempiricallyevaluatedagainstapanelofIQFDiUbsubstratestoselectthetheoptimalfluorophore/quencherpairing.

Inordertorapidlydeterminewhichofthesefluorophore/quencherpairsareoptimalforyourDUBand/ordeterminedlikagespecificityforyourDUB,LifeSensorsoffersapanelof6substratescomprisedofeachK48-linkedsubstrateavailabletodate.

Needbulkquantities?Wewouldbehappytoworkoutacustompricethatfitsyourbudget.Pleaseemail info@lifesensors.com formoreinformation.

ThisproductiscoveredbyUSpatent#8,518,660.Bypurchasingthisproduct,thepurchaseragreestocomplywiththetermsofourLimitedUseLabelLicensefoundintheproductmanual.

Info

Amount	25uLof20uMpereachsubstrate
State	Liquid
Linkage	K48
Buffer	50mMSodiumMES,pH6.0
Variants	K48-(1-6)
Fluorescentreadout	TAMRA
Storageinformation	IQFDiUbsarestablefor4-6monthsat4ºC.Longterm(>6months)storageat-80ºCisrecommended.Avoidrepeatedfreeze/thawcycles.
DiUb48	DiUb48-1, DiUb48-2, DiUb48-3, DiUb48-4, DiUb48-5, DiUb48-6

Applications

DeterminationoftheoptimalDiUb48-linkedsubstrateforyourDUB
Kineticcharacterizationofdeubiquitylases(DUBs)
DemonstrationofnovelDUBactivityand/orlinkagespecificity
MonitoringofheterologousDUBproduction/purification
Additionalmaterialsrequired(forfurtherdetails,pleaseseemanual):
- 384-(GreinerBioOne781209) or96-well (655076)blackplatesorequivalent
- Fluorescentplatereader
- FiltersormonochromatorsformonitoringfluorescenceofTAMRA(Ex540nm,Em580nm)
- Dichroicmirrorwitha550-570nmcutoffisrecommendedforoptimalsignal-to-background

Benefits

RapidandconvienentdeterminationoftheoptimalDiUbsubstrateforyourDUB
MoreBIOLOGicallyrelevantsubstratecomparedtotrADItionalmono-ubiquitinderivatives
FluorophoresareNOTattheN-orC-terminus,modificationsknowntoperturbubiquitinstructure
Avarietyoflinkages andfluorophorepositioningavailableforoptimalactivityofyourDUBofinterest
Validatedinboththe96-welland384-wellformat

Documents

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LifeSensors/DU0101-K48 PANEL/DU0101-K48 PANEL