Recombinantubiquitinhavingasinglesubstitutionofarginineforlysineatposition29.
UbiquitinisasmallpolypeptidethatcanbeconjugatedviaitsC-terminustoaminegroupsoflysineresidueontargetproteins.Thisconjunctionisreferredtoasmonoubiquitylation.Additionalubiquitinmoietiescanbesubsequentlyconjugatedtothisinitialubiquitin,utilizinganyone(ofseven)lysineresiduesonthesurfaceofubiquitin.Theformationoftheseubiquitinchainsisreferredtoaspolyubiquitylation.
Info
Source | Humanrecombinant |
Form | liquid |
Molecularweight | 8,592Da |
Purity | >=95%byRP-HPLC |
AffinityTag | None |
Concentration | 4mg/mL |
Buffer | 20mMTris,pH7.5,0.15MNaCl,10%glycerol |
Storage | Longtermstorageat-80°C.Avoidmultiplefreeze/thawcycles. |
StABIlity | 1yearunderrecommendedstorageconditions |
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