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LifeSensors/SI207-1MG Ubiquitin-K48/SI207-1MG Ubiquitin-K48

UbiquitinisasmallpolypeptidethatcanbeconjugatedviaitsC-terminustoaminegroupsoflysineresidueontargetproteins.Thisconjunctionisreferedtoasmonoubiquitylation.Additionalubiquitinmoietiescanbesubsequentlyconjugatedtothisinitialubiquitin,utilizinganyone(ofseven)lysineresiduesonthesurfaceofubiquitin.Theformationoftheseubiquitinchainsisreferredtoaspolyubiquitylation.

Thistag-freerecombinantformofhumanubiquitinisengineeredtohaveallavailablelysinesmutatedtoarginines,exceptatposition48. Thismolecule,therefore,canonlyformpolyubiquitinchainsofK48linkagetype.



Info

SourceHumanrecombinant
Formliquid
Molecularweight8,732Da
Purity>=95%bySDS-PAGE
AffinityTagNone
Concentration4mg/mL
Buffer20mMTris,pH8.0,0.15MNaCl,10%glycerol
Storage-80°C,avoidcyclesoffreeze/thaw
StABIlity1yearunderrecommendedstorageconditions


Applications
  • CharacterizationofproteinsresponsIBLeforubiquitinconjugationorremoval.
  • Ligandbindingstudieswithubiquitinrelatedproteins.
  • Characterizationoflinkagetypefortargetprotein.
  • ProfilingofE2/E3interactions.
  • CharacterizationofE3polyubiquitylationactivity.
  • Benefits: Native(tagfree)recombinantwild-typeubiquitin.


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