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ORDERINGINFORMATION
CatalogNo.:43038
Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.
BACKGROUND
Elk-1isamemberoftheEtsfamilyoftranscriptionfactorsandoftheternarycomplexfactor(TCF)subfamily.ProteinsoftheTCFsubfamilyformaternarycomplexbybindingtotheserumresponsefactorandtheserumreponseelementinthepromoterofthec-fosproto-oncogene.Theproteinencodedbythisgeneisanucleartargetfortheras-raf-MAPKsignalingcascade.
Alternativelysplicedtranscriptvariantsencodingthesameproteinhavebeenfoundforthisgene.
SPECIFICATIONSUMMARY
Antigen:Peptidesequencethatincludesphosphorylationsiteofthreonine417(L-S-T(p)-P-V)derivedfromhumanElk1andconjugatedtoKLH.
Accessionno.:P19419,NP_001244097.1
HostSpecies:Rabbit
Specificity:Thisantibodydetectsendogenoushuman,mouse,andratElk1onlywhenphosphorylatedatthreonine417.
APPLICATION
Immunohistochemistry:useatdilutionof1:50-1:100.
Immunofluorescence:useatdilutionof1:100-1:200.
DetectionofElk1(phospho-Thr417)inparaffin-embeddedhumanbreastcarcinomatissue.
DetectionofElk1(phospho-Thr417)inmethanol-fixedHeLacells.
Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-termuse.
Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.
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