Neutralizing IgG monoclonal antibody to human CD14

Anti-hCD14-IgG is a chimeric monoclonal antibody specific for human CD14. It has been selected for its ability to efficiently neutralize the biological activity of TLR2 or TLR4.

CD14 is a glycosylphosphatidylinositol (GPI)-anchored membrane protein that acts as a bacterial pattern recognition receptor [1]. It is found on cells derived from the monocyte/macrophage lineage, as well as neutrophils and B lymphocytes. CD14 serves as a member of the heteromeric lipopolysaccharide (LPS) receptor complex that also contains TLR4 and MD2 [2]. It binds LPS but is not capable of initiating a transmembrane activation signal since it does not contain a cytoplasmic domain. Upon LPS binding, CD14 physically associates with TLR4 which in turn transduces the signal. CD14 was also shown to interact with TLR2 in response to various microbial infections [3].

Anti-hCD14-IgG was generated by recombinant DNA technology. It has been produced in CHO cells and purified by affinity chromatography.

References:

1. Pugin J. et al., 1994. CD14 is a pattern recognition receptor. Immunity. 1(6):509-16.2. Da Silva Correia J. et al., 2001. Lipopolysaccharide is in close proximity to each of the proteins in its membrane receptor complex. transfer from CD14 to TLR4 and MD-2. J Biol Chem. 276(24): 21129-35.3. Aderem A. & Ulevitch R.J. , 2000. Toll-like receptors in the induction of the innate immune response. Nature. 406(6797):782-7.

Specifications

Clonality: Monoclonal antibody

Applications: Neutralizing TLR2 or TLR4-induced cellular activation. It can also be used for flow cytometry.

Specificity: Human CD14

Clone: D3B8

Isotype: Human IgG1, kappa

Formulation: 0.2 μm filtered solution in sodium phosphate buffer with glycine, saccharose and stabilizing agents

Contents

• 100 μg purified anti-hCD14-IgG provided azide-free and lyophilized.

Product is shipped at room temperature.

Store lyophilized antibody at -20 °C.

Product is stable for 1 year.

Description

CD14 is a glycosylphosphatidylinositol (GPI)-anchored membrane protein which acts as a bacterial pattern recognition receptor [1]. CD14 is found on cells derived from the monocyte/macrophage lineage, as well as neutrophils and B lymphocytes. CD14 serves as a member of the heteromeric lipopolysaccharide (LPS) receptor complex that also contains TLR4 and MD2 [2]. CD14 binds LPS but is not capable of initiating a transmembrane activation signal since it does not contain a cytoplasmic domain. Upon LPS binding, CD14 physically associates with TLR4 which in turn transduces the signal. CD14 was also shown to interact with TLR2 in response to various microbial infections [3].

Pugin J. et al., 1994. CD14 is a pattern recognition receptor. Immunity. 1(6):509-16.Da Silva Correia J. et al., 2001. Lipopolysaccharide is in close proximity to each of the proteins in its membrane receptor complex. transfer from CD14 to TLR4 and MD-2. J Biol Chem. 276(24): 21129-35.Aderem A. & Ulevitch R.J. , 2000. Tolllike receptors in the induction of the innate immune response. Nature. 406(6797):782-7.