Nordicmubio/Rabbit anti Human Actin, (cytoplasmic 1) (NT)/X2742P

Isotype	Ig
ProductType	AntigenImmunoaffinityPurifiedPolyclonal
Units	100µg
Host	Rabbit
Speciesreactivity	Human
Application	ImmunohistochemistryWesternBlotting

BackgroundActinsarehighlyconservedproteinsthatareinvolvedincellmotility,structureandintegrity,processesthatarecrucialfortissuedevelopmentandthedevelopmentoforganism.TheactinCytoskeletonisoneoftheprincipaldriversofcellmotilityandiscapableofrespondingtocomplexsignalingcascades.Recentevidencesuggeststhatitmayplaykeyrolesinregulatingapoptosisandaging.Betaactinisoneofsixdifferentactinisoformswhichhavebeenidentified.LikeGAPDH,b-actinisconstitutivelyexpressedathighlevelsinalmostalltissuesandcelllinesmakingitidealforuseasaloADIngcontrolMarkerinimmunoblots.

Source

Immunogen:SyntheticpeptidederivedfromtheN-terminalofthebeta-actinprotein.

Product

ProductForm:AffinityPurified

Formulation:Providedassolutioninphosphatebufferedsalinewith0.08%sodiumazide

PurificationMethod:AntigenImmunoaffiinityPurification

Concentration:Seevialforconcentration

ApplicationsAntibodycanbeusedforWesternblotting(1-5µg/ml)andimmunohistochemsitryonparaffin-embeddedtissues(10µg/ml),Optimalconcentrationshouldbeevaluatedbyserialdilutions.

FunctionalAnalysis:WesternBlotting

PositiveControl:Humanlivertissue

StorageProductshouldbestoredat-20ºC.Aliquottoavoidfreeze/thawcycles

ProductStABIlity:Productsarestableforoneyearfrompurchasewhenstoredproperly

ShippingConditions:Shipatambienttemperature,freezeuponarrival

CautionThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Itmaycontainhazardousingredients. PleaserefertotheSafetyDataSheets(SDS)foradditionalinformationandproperhandlingprocedures.Disposeproductremaindersaccordingtolocalregulations.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-Mubio​acceptsnoliabilityforanyinaccuraciesoromissionsinthisinformation.

References1.PonteP.,NgS.Y.,EngelJ.,GunningP.,KedesL.,EvolutionaryconservationintheuntranslatedregionsofactinmRNAs:DNAsequenceofahumanbeta-actinCDNA.NucleicAcidsRes.12:1687-1696(1984)2.Nakajima-IijimaS.,HamadaH.,ReddyP.,KakunagaT.,Molecularstructureofthehumancytoplasmicbeta-actingene:interspecieshomologyofsequencesintheintrons.Proc.Natl.Acad.Sci.U.S.A.82:6133-6137(1985)3.ProcaccioV.,SalazarG.,OnoS.,StyersM.L.,GearingM.,DavilaA.,JimenezR.,JuncosJ.,GutekunstC.-A.,MeroniG.,FontanellaB.,SontagE.,SontagJ.-M.,FaundezV.,WainerB.H.Amutationofbeta-actinthataltersdepolymerizationdynamicsisassociatedwithautosomaldominantdevelopmentalmalformations,deafness,anddystonia.Am.J.Hum.Genet.78:947-960(2006)[PubMed:16685646][Abstract]

ProteinReference(s)

DatabaseName:UniProt

Accessionnumber:P60709

SpeciesAccession:Human

SafetyDatasheet(s)forthisproduct:NM_SodiumAzide/wp-content/uploads/SDS/AntibodySDSwithSodiumAzideNoridic-MUbio.pdf