Nordicmubio/Rabbit anti Human Beta-secretase 1 (CT)/X1135P

Isotype	IgG
Product Type	Polyclonal Antibody
Units	100 µg
Host	Rabbit
Species reactivity	HumanMouse
Application	Western Blotting

BackgroundAccumulation of the amyloid-(A ) plaque in the cerebral cortex is a critical event in the pathogenesis of AlzheimerÕs disease. Apeptide is generated by proteolytic cleavage of the amyloid protein precursor (APP) at- and-sites by two proteases. APP is first cleaved by secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for secretase to generate the 4 kDa amyloid-peptide, which is deposited in the brains of all suffers of AlzheimerÕs disease. The long-sought secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2)1-4.BACE/Asp2 is a novel transmembrane aspartic protease and colocalizes with APP.

Synonyms: BACE, KIAA1149, Beta-site amyloid precursor protein cleaving enzyme 1

Source

Immunogen: Synthetic peptide corresponding to amino acids 485 to 501 of human BACE.Sequence differs from that of rat and mouse by one amino acid.

Product

Product Form: Unconjugated

Formulation: Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative

Purification Method: Antigen Immunoaffiinity Purification

Concentration: See vial for concentration

ApplicationsDetects BACE by Western blot at 0.5 to 1 µg/ml.Detects an approximately 70 kDa band in human brain tissue lysate.Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Human brain tissue lysate

StorageProduct should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Stability: Products are stable for one year from purchase when stored properly

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References1. Vassar, R., et al. Beta-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE. Science 1999, 286, 735-7412. Hussain, I., et al.Identification of a novel aspartic protease (Asp 2) as beta-secretase. Mol. Cell Neurosci. 1999, 14, 419-4273. Yan, R., et al. Membrane-anchored aspartyl protease with Alzheimer's disease beta-secretase activity. Nature 1999, 402, 533-5374. Sinha, S., et al. Purification and cloning of amyloid precursor protein beta-secretase from human brain. Nature 1999, 402, 537-540

Protein Reference(s)

Database Name: UniProt

Accession number: P56817 (Human)P56818 (Mouse)

Species Accession: Human

Safety Datasheet(s) for this product:NM_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium Azide Noridic-MUbio.pdf