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| Isotype | IgG2a |
| ProductType | Single-ColorNegativeControl |
| Units | 100tests |
| Host | Mouse |
| Application | FlowCytometry |
BackgroundNegativecontrolforimmunofluorescencestainingwithFITCconjugatedmousemonoclonalantibodies.Assessmentofnon-specificbindingofmousemonoclonalantibodiestohumancellsurfaceantigens.
ProductPurifiedmouseIgG2aconjugatedtoFITC,assolutioninphosphatebufferedsalinewith0.08%sodiumazideand0.2%carrierprotein
Formulation:Providedassolutioninphosphatebufferedsalinewith0.08%sodiumazideand0.2%carrierprotein
PurificationMethod:ProteinA/GChromatography
Concentration:Seevialforconcentration
ApplicationsConsulttheappropriatefactsheettodeterminetheamountofantibodiestobeusedasacontrolforPBMCorWholeBlood.PBMC:Add10μlofMAB/10^6PBMCin100μlPBS.Mixgentlyandincubatefor15minutesat2ºto8ºC.WashtwicewithPBSandanalyze.WHOLEBLOOD:Add10μlofMAB/100μlofwholeblood.Mixgentlyandincubatefor15minutesatroomtemperature20ºC.Lysethewholeblood.WashoncewithPBSandanalyze.Seeinstrumentmanufacturer’sinstructionsforLysedWholeBloodandImmunofluorescenceanalysiswithaflowcytometerormicroscope.
WorkingConcentration:Titeredforflowcytometry
StorageProductshouldbestoredat4-8ºC.DONOTFREEZE.Storeprotectedfromlight.Reagentisstablefortheperiodshownonthevialwhenstoredproperly.
ProductStABIlity:Productsarestableforoneyearfrompurchasewhenstoredproperly
ShippingConditions:RoomTemperature
CautionThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Itmaycontainhazardousingredients. PleaserefertotheSafetyDataSheets(SDS)foradditionalinformationandproperhandlingprocedures.Disposeproductremaindersaccordingtolocalregulations.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-Mubioacceptsnoliabilityforanyinaccuraciesoromissionsinthisinformation.
ProteinReference(s)
Accessionnumber:SwissProtP01863
WarrantyReagentisstablefortheperiodshownonthevialwhenstoredproperly.
SafetyDatasheet(s)forthisproduct:NM_SodiumAzide/wp-content/uploads/SDS/AntibodySDSwithSodiumAzideNoridic-MUbio.pdf
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