Nordicmubio/Rabbit anti Human Beta-secretase 2 (CT)/X1137P

Isotype	IgG
Product Type	Polyclonal Antibody
Units	100 µg
Host	Rabbit
Species reactivity	Human
Application	Western Blotting

BackgroundAccumulation of the amyloid-(A ) plaque in the cerebral cortex is a critical event in the pathogenesis of AlzheimerÕs disease. Apeptide is generated by proteolytic cleavage of the amyloid protein precursor (APP) at- and-sites by proteases. The long-sought secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2)1-4. A BACE homolog was recently cloned and designated BACE2, Asp1, DRAP (for Down region aspartic protease), and memapsin 14-9. BACE2 also cleaves APP at-site and at a different site within A 8. BACE2 locates on chromosome 21q22.3, the so-called ÔDown critical regionÕ, suggesting that BACE2 and Amay also contribute to the pathogenesis of Down syndrome6,7

Synonyms: AEPLC, ALP56, ASP21, Rabbit anti-β site APP (Amyloid β precursor protein) Cleaving Enzyme-2 (C-Terminal),

Source

Immunogen: Synthetic peptide corresponding to amino acids 496 to 511 of human BACE2

Product

Product Form: Unconjugated

Formulation: Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative

Purification Method: Antigen Immunoaffiinity Purification

Concentration: See vial for concentration

ApplicationsDetects BACE2 by Western blot at 0.5 to 1 µg/ml.Detects an approximately 70 kDa band in human heart tissue lysate.Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Human heart tissue lysate

StorageProduct should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Stability: Products are stable for one year from purchase when stored properly

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References1. Vassar, R., et al. Beta-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE. Science 1999, 286, 735-7412. Hussain, I., et al.Identification of a novel aspartic protease (Asp 2) as beta-secretase. Mol. Cell Neurosci. 1999, 14, 419-4273. Yan, R., et al. Membrane-anchored aspartyl protease with Alzheimer's disease beta-secretase activity. Nature 1999, 402, 533-5374. Sinha, S., et al. Purification and cloning of amyloid precursor protein beta-secretase from human brain. Nature 1999, 402, 537-5405. Lin, X., et al. Human aspartic protease memapsin 2 cleaves the beta-secretase site of beta-amyloid precursor protein. Proc. Natl. Acad. Sci. USA 2000 15, 97, 1456-14606. Acquati, F., et al. The gene encoding DRAP (BACE2), a glycosylated transmembrane protein of the aspartic protease family, maps to the down critical region. FEBS Lett. 2000, 468, 59-647. Solans, A., et al. A new aspartyl protease on 21q22.3, BACE2, is highly similar to Alzheimer's amyloid precursor protein beta-secretase. Cytogenet. Cell Genet. 2000, 89, 177-1848. Farzan, M., et al. BACE2, a beta -secretase homolog, cleaves at the beta site and within the amyloid-beta region of the amyloid-beta precursor protein. Proc. Natl. Acad. Sci. USA 2000, 97, 9712-97179. Bennett, B.D., et al. Expression analysis of BACE2 in brain and peripheral tissues. J. Biol. Chem. 2000, 275, 20647-20651

Protein Reference(s)

Database Name: UniProt

Accession number: Q9Y5Z0

Species Accession: Human

Safety Datasheet(s) for this product:NM_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium Azide Noridic-MUbio.pdf