Fluorescence Bottom Reading for Cell-Based Assays

Summary:

Cell-based applications are central to life science research. They may range from cytotoxicity, proliferation, apoptosis and GPCR signaling assays to high-throughput screening (HTS) drug discovery applications.
Adherent cell types are typically analyzed through the well bottom in order to bring the cell monolayer as close as possible to the detector and avoid unspecific fluorescence noise by the overlying growth medium. For this reason it is essential to guarantee illumination and reading of the entire well bottom, since cells are not always homogeneously distributed over the growth surface.
the present application note is to assess the performance of the improved, fluorescence bottom fiber of the new Infinite 200 PRO in the detection of GFP-transfected cells. Measurement characteristics like well-to-well uniformity and reading reproducibility were investigated.

Attachment:

Fluorescence Bottom Reading for Cell-Based Assays.pdf