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ARBOR ASSAYS/Allopregnanolone EIA Kit, Monoclonal Antibody Based/K061-H5/H5188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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ARBOR ASSAYS/Allopregnanolone EIA Kit, Monoclonal Antibody Based/K061-H5/H5

  • MostSensitive
  • MultiSpecies
  • USE-Measureallopregnanoloneinavarietyofsamples
  • SAMPLE-Urine,TCMorExtractedSerum,Plasma,andDriedFeces
  • SAMPLES/KIT-39or231inDuplicate
  • SENSITIVITY-129.7pg/mL
  • SELECTIVE-LowCrossReactivitytoProgesteroneandMetabolites

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AllopregnanoloneaidsneurogenesisandhasbeenfoundtoreverseneuronproliferativedeficitandcognitivedeficitsinamousemodelofAlzheimer’sdisease. AllopregnanolonehasalsobeenshowntorestorefunctionalityinamousemodelofParkinson’sdiseaseandtoimprovebehavioralproblemsinpost-traumaticstressdisorder.

TheDetectX®AllopregnanoloneImmunoassaykitisdesignedtoquantitativelymeasureallopregnanolonepresentinextractedserum,plasma,driedfecalsamples,ordilutedurineandtissueculturemediasamples. Pleasereadthecompletekitinsertbeforeperformingthisassay. Anallopregnanolonestandardisprovidedtogenerateastandardcurvefortheassayandallsamplesshouldbereadoffthestandardcurve. StandardsordilutedsamplesarePipettedintoaclearmicrometerplatecoatedwithanantibodytocapturemouseantibodies. Anallopregnanolone-peroxidaseconjugateisaddedtothestandardsandsamplesinthewells. Thebindingreactionisinitiatedbytheadditionofamonoclonalantibodytoallopregnanolonetoeachwell.

Thekithastwoformatoptions:aprimaryincubationoftwohoursatroomtemperaturewithshaking,orovernightat4°C. Attheendoftheincubationperiodtheplateiswashedandsubstrateisadded. Thesubstratereactswiththeboundallopregnanolone-peroxidaseconjugate.Afterashortincubation,thereactionisstoppedandtheintensityofthegeneratedcolorisdetectedinamicrotiterplatereadercapableofmeasuring450nmwavelength. Theconcentrationoftheallopregnanoloneinthesampleiscalculated,aftermakingsuitablecorrectionforthedilutionofthesample,usingsoftwareavailablewithmostplatereaders.

 k061-h

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