Summary:
Cell-basedapplicationsarecentraltolifescienceresearch.Theymayrangefromcytotoxicity,proliferation,apoptosisandGPCRsignalingassaystohigh-throughputscreening(HTS)drugdiscoveryapplications.
AdherentcelltypesaretypicallyanalyzedthroughthewellbottominordertobringthecellmonolayerascloseaspossIBLetothedetectorandavoidunspecificfluorescencenoisebytheoverlyinggrowthmedium.ForthisreasonitisessentialtoguaranteeIlluminationandreADIngoftheentirewellbottom,sincecellsarenotalwayshomogeneouslydistributedoverthegrowthsurface.
thepresentapplicationnoteistoassesstheperformanceoftheimproved,fluorescencebottomfiberofthenewInfinite200PROinthedetectionofGFP-transfectedcells.Measurementcharacteristicslikewell-to-welluniformityandreadingreproducibilitywereinvestigated.
Attachment:
FluorescenceBottomReadingforCell-BasedAssays.pdf