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ARBOR ASSAYS/Aldosterone Chemiluminescent Immunoassay Kit/K052-C5/C5

  • MostSensitive
  • MultiSpecies
  • SENSITIVE-Measurefrom1,500to2.06pg/mL
  • SAMPLE-Serum,EDTAorHeparinPlasma,Urine,FecalExtractsandTCM
  • SAMPLES/KIT-39or231inDuplicate
  • STABILITY-Liquid4˚CStableReagents

TheDetectX®AldosteroneChemluminescentImmunoassay(CLIA)kitisdesignedtoquantitativelymeasureAldosteronepresentinextractedserumandplasma,orinurine,extracteddriedfecalsamples,andtissueculturemediasamples. Analdosteronestocksolutionisprovidedtogenerateastandardcurve.Thekithasastandardrangefrom1,500to2.06pg/mL. SamplesarePipettedintoawhitemicrotiterplatecoatedwithanantibodytocapturesheepantibodies.Analdosterone-peroxidaseconjugateisaddedtothestandardsandsamplesinthewells.Thebindingreactionisinitiatedbytheadditionofapolyclonalantibodytoaldosteronetoeachwell.Afterincubationovernightat4°C,theplateiswashedand chemiluminescentsubstrateisadded.Thesubstratereactswiththeboundaldosterone-peroxidaseconjugate.Thesubstratereactswiththeboundcorticosterone-peroxidaseconjugatetoproducelight.

K052-C

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