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ARBOR ASSAYS/Butyrylcholinesterase Fluorescent Activity Kit/K016-F1/F1

  • MostSensitive
  • MultiSpecies
  • USE-MeasureBChEActivityin20MinutesorKinetically
  • SAMPLE-Serum,Plasma,andCSF
  • SAMPLES/KIT-88inDuplicate
  • SENSITIVE-Measure<0.02=""mu/ml=""of=""bche="">
  • STABILITY-AllReagentsStableat4˚C

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TheDetectX®Butyrylcholinesterase(BChE)FluorescentActivitykitisasimple,sensitiveandstablekitthatmeasuresBChEactivityinawidevarietyofsamples.Thekitcontainseverythingneededtomeasureactivity,includingthemicrotiterplate.Dilutedsamplesareaddedtothewells.ThereactionisinitiatedbyadditionofareactionmixcontainingthebutyrylthiocholinesubstrateandThioStar®.Aftera20minuteincubation,thesignalisreadat510nmwithexcitationat390nm.Theactivitycanalsobereadkinetically.AstablehumanBChEstandardsolutionisprovidedtogenerateastandardcurvefortheassay.TheassayshowsalmostzeroreactivitywithAChE.

Butyrylcholinesterase(BChE)belongstothesamestructuralclassofproteinsasacetylcholinesterase(AChE).The440kDatetramericglycoproteinispredominantlyfoundinblood,kidneys,intestine,liver,lung,heartandthecentralnervoussystem.Manyspecies,suchashuman,horseandmiceexhibithighBChEactivityinplasma,whereasratshavehigheracetylcholinesteraseactivityinplasma.AdeficiencyofBChEcanresultindelayedmetabolismofvariousdrugs,suchascocaine,andtreatmentwithdosesofBChEcanhelpinovercomingthephysiologicalreactiontothem.InAlzheimer’sdiseasetherapeutics,theuseofpotentcholinesteraseinhibitorsprotectthelimitedacetylcholinelevels,actingonbothAChEandBChE.BChEmayhaverolesinattention,executivefunction,emotionalmemoryandbehaviour.Otherintentionalorenvironmentalmethodsofimpairmentareorganophosphatesandcarbamateswithanticholinergicproperties,whichareusedasinsecticidesworldwideoraswarfareagents.

Butyrylcholinesterase Fluorescent Activity Standard Curve

DataReductionTemplatebyMyAssays

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