Single Tube Custom TaqMan® Gene Expression Assays

CustomTaqMan®ExpressionAssaysarethecustomversionofourpredesignedreal-timePCR5"nucleaseTaqMan®AssaysforrelativequantificationofRNAexpression.Simplyprovideuswithyourtargetsequenceofinterest,orsubmitprimer/probesequencesofyourowndesign,andsoonyou"llbereadytoperformRNAexpressionstudiesonanycodingornon-codingRNAinanyorganism.

HowtoOrder

Reviewour DesignandOrderingGuide(PDF) forbestresults.
Usethe CustomTaqMan®AssayDesignTool toinputsequences,submitfordesign,andorderCustomAssays.

Eachcustomassayisamixofforwardprimer,reverseprimer,andFAM™dye-labeledTaqMan®MGBprobe.Designedtorununderuniversaloligoconcentrationandthermalcyclingconditionsfortwo-stepRT-PCR,theseassaysaresimpletouse.JustaddTaqMan®MasterMixandyourCDNAsampletogeneratesensitive,reproducIBLe,andtrulyquantitativeRNAexpressiondata.

CustomTaqMan®AssayDesignTool

ComparisonofCustomandCustomPlusTaqMan®GeneExpression
Assayproducts

TherearetwocustomassayoptionstomeetyourRNAresearchneeds:CustomTaqMan®GeneExpressionAssaysandCustomPlusTaqMan®RNAAssays.BothallowyoutosubmitatargetsequencetoourTaqMan®Assaydesignpipeline,whichusesyourinputsequencetodesigncustomprimerandprobesequences.Seethekeydifferencesbetweenthetwooptionsbelow:

Feature	CustomPlusTaqMan®RNAAssays	CustomTaqMan®GeneExpressionAssays
Bioinformaticanalysisperformedoninputtargetsequences
Insilico QCperformedonassaysequences
Specificityoptionforgene-levelortranscript-leveldetection
ContainsTaqMan®FAM™-MGBprobeand2unlabeledprimers
Runsunderuniversalthermalcyclingconditions
Assaysequencesprovided
Availableforanyspecies	*Availableforanyspecieswithgenomeinformationinourdatabase
AutomaticcheckforpredesignedTaqMan®Assayswhichmaydetectinputsequence

CustomPlusTaqMan®RNAAssaysaredesignedusingthefullbioinformaticpoweroftheTaqMan®Assaydesignpipeline,whichusesproprietaryalgorithmsthathavegeneratedmillionsofassaydesignsandisregardedasthebenchmarkintheindustryforqPCRdesign.IfyoudonotneedbioinformaticsorQCchecksperformedonyoursequences,thenourstandardCustomTaqMan®GeneExpressionAssayswillmeetyourneeds.

EasyOrdering

TargetsequencesubmissioniseasyusingtheCustomTaqMan®AssayDesignTool(CADT).Simplyselectyourbioinformaticanalysisandassayspecificitypreferences,andenteryourtargetsequence.Sequencesubmissionsarecompletelyconfidential;LifeTechnologieswillnotshareyourtargetsequencesorassaysequenceswithanythirdparties.

Ifyoudonothaveapre-definedinputsequence,theCADTwillhelpyoutosearchforsequencesbykeyword(genesymbol,accessionnumber,etc.)orbygenomiclocationinabroadrangeofthemostpopularandwell-studiedmodelspeciesrangingfromhumanandmousetopig,dog,cow, ArABIdopsis,andmore.

CustomTaqMan®AssayDesignTool

Orderinginformation

Sku	ProductName	Size
4331348	CustomTaqMan®GeneExpressionAssay,SM	360reactions,20x
4332078	CustomTaqMan®GeneExpressionAssay,MED	750reactions,20x
4332079	CustomTaqMan®GeneExpressionAssay,LG	2900reactions,60x
4441114	CustomPlusTaqMan®RNAAssay,SM	360reactions,20x
4441117	CustomPlusTaqMan®RNAAssay,MED	750reactions,20x
4441118	CustomPlusTaqMan®RNAAssay,LG	2900reactions,60x

References

BergmannOetal.(2011)Identificationofcardiomyocytenucleiandassessmentofploidyfortheanalysisofcellturnover.ExpCellRes 2,188-194.
HevirN,SinkovecJ,RiznerTL(2011)DisturbedexpressionofphaseIandphaseIIestrogen-metabolizingenzymesinendometrialcancer:lowerlevelsofCYP1B1andincreasedexpressionofS-COMT. MolCellEndocrinol 1,158-167.
ColazzoFetal.(2011)ExtracellularmatrixproductionbyADIpose-derivedstemcells:implicationsforheartvalvetissueengineering. Biomaterials 1,119-127.

Productliterature

FactSheet:TaqMan®Assayambientshipping
WhitePaper:TaqMan®Assaysambientshipping
WhitePaper:Thedesignprocessforanewgenerationofquantitativegeneexpressionanalysistools:TaqMan®probe-basedassaysforhuman,mouse,andratgenes
DesignandOrderingGuide:CustomTaqMan®Assays
UserProtocol:TaqMan®GeneExpressionAssays
QuickReferenceCard:TaqMan®GeneExpressionAssays

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Single Tube Custom TaqMan® Gene Expression Assays