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Human IgE for Mast Cell Degranulation Assays
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HumanIgEforMastCellDegranulationAssays
 
Cat.No. 250206 HumanIgE(biotinylated) issuccessfullyusedtosensitizehumanmastcellsandtriggerdegranulationwhenstreptavidinisaddedtothecellculturemedia.HumanIgEproductshave been usedtostimulate: 
  • humanmastcells
  • humanairwaysmoothmuscle cells(HASM)
  • humanbronchial/trachealsmoothmuscle cells(B/TSM)
  • acutemyelogenousleukemiablasts(AML)
  • humanneutrophils
 
BiotinylatedhumanIgEandstreptavidintriggerdegranulation of LAD2humanmastcells.LAD2cellsweresensitizedovernightwith0.1ug/mlbiotinylatedhumanIgE(AbbiotecCat.No.250206).Cellswereincubatedwithstreptavidin(SA)for30minat37°C.Degranulationwasmonitoredbybeta-hexosaminidasereleaseintothesupernatantcalculatedasapercentageoftotalcontent.
DatakindlyprovidedbyProf.FernandodeMora,Universidad Autonoma de Barcelona,Spain.
 
HumanIgE

Cat.No.250202 isa non-immunehumanIgEproducedinvitrobya monoclonalhybridomaisolatedfromahealthyhumandonor.TheIgEisan affinitypurifiedfullyhumanantibody withepsilonheavychainandkappalightchain.ThemonoclonalsourceofIgEensuresantibodypropertyconsistencyandotherIgisotypecontaminationfree.HumanIgEproductscanbe usedas standardsinquantitativeIgEassaysandcellsensitizationassays.AbbiotecoffershumanIgEinpurified,azidefreeandbiotinylatedformats.

Contactour CustomerServiceDept forcomprehensivelisting,availABIlity,andnewproductstobereleased. DownloadtheHumanIgEandSecondaryAntibodiesBrochure here.

QualitycontrolofHumanIgEpreparation  fromhybridoma(lane2)vs.IgEsamplepurifiedfrommyelomapatientserum(lane3).MolecularweightMarkersareshowninlane1. 

Quality control of human IgE preparation from hybridoma (lane 2) vs. IgE sample purified from myeloma patient serum (lane 3). MW markers are shown in lane 1.
ControlandSecondaryAntibodies

Browseourcatalogforhuman,mouse,rabbitandratantibodiesthataredevelopedforELISA,westernblot,immunohistochemistryandflowcytometryapplications. Isotypecontrols,humanIgEandsecondaryantibodies arelistedbelow.

Cat.No.ProductName
250202HumanIgE,0.1mg
250204HumanIgE,1mg
250203HumanIgE,azidefree,0.1mg
250205HumanIgE,azidefree,1mg
250206HumanIgE,biotin-conj.,0.1mg
252240Anti-goatIgGAntibody(HRP),0.5mg
250114Anti-humanIgAAntibody,0.1mg
250140Anti-humanIgEAntibody,0.1mg
250116Anti-humanIgGAntibody,0.1mg
250928Anti-humanIgMAntibody,0.1mg
250929Anti-humanIgMAntibody(PE),2ml
250930Anti-humanIgMAntibody(FITC),2ml
254517Anti-humanIgMAntibody(HRP),0.1mg
250987Anti-humankappaIgAntibody,0.1mg
251043Anti-humankappaIgAntibody(FITC)2ml
251099Anti-humankappaIgAntibody(PE),2ml
252241Anti-humanlamBDaIgAntibody,0.1mg
252242Anti-humanlambdaIgAntibody(FITC)
252243Anti-humanlambdaIgAntibody(PE)2ml
250137Anti-mouseIgGAntibody(HRP),1mg
250138Anti-mouseIgGAntibody (FITC),1mg
250139Anti-mouseIgGAntibody(Fab’)2 (FITC)
252237Anti-rabbitIgGAntibody(HRP),1mg
252238Anti-rabbitIgGAntibody(FITC),1mg
250842Anti-ratIgAAntibody,0.1mg
250115Anti-ratIgGAntibody(FITC),0.1mg
252239Anti-ratIgGAntibody(HRP),1mg
250446Anti-ratkappaIgAntibody,0.2mg
250198ControlMouseIgG1,0.1mg
250207ControlMouseIgG1(PE),2ml
250199ControlMouseIgG2a,0.1mg
252507ControlMouseIgG2a(FITC),2ml
252508ControlMouseIgG2a(PE),2ml
252509ControlMouseIgG2b,0.1mg
252510ControlMouseIgG2b(FITC),2ml
252511ControlMouseIgG2b(PE),2ml
254513ControlRabbitIgG,10mg
 
SPECIALOFFER FOROURSUBSCRIBERS! UntilMay31,2015 -Save10%onHumanIgE,SecondaryandControl Antibodyproducts. UsePromoCode ENEWS0415 attimeoforder. Termsandconditionsapply.

LATESTPRODUCTCITATIONS 
Foracomprehensive listofproductcitations,refertothe citationwebpage.

Cat.No.250202HumanIgE fornewassaydevelopment
ChangC.etal.2014.Analyst. PMID24821053.Label-freecolorimetricaptasensorforIgEusingDNApseudoknotprobe.

Cat.No. 250203 HumanIgEAzideFree formastcell sensitization
MillsK.etal.2014.Allergy. PMID24697338.VitaminE,gamma-tocopherol,diminishesexvivobasophilresponsetodustmiteallergen.

Cat.No.250205HumanIgEAzideFree(1mg) forIgEreceptorbindingstudies
SharquieI.etal.2013.BMCImmunol. PMID24330349.AninvestigationintoIgE-facilitatedallergenrecognitionandpresentationbyhumandendriticcells.

Cat.No. 250206 HumanIgE (biotinylated)formastcelldegranulationstudies
Ainsua-EnrichE.etal.2012.J Immunol. PMID 22896635.TheAdapter3BP2IsRequiredforEarlyandLateEventsinFcεRIsignalinginHumanMastCells.

JansonC.etal.2012.PLoSONE. PMID 23185384. RIN3IsaNegativeRegulatorofMastCellResponsestoSCF.

Serra-PagesM.etal.2012.J Leukoc Biol. PMID 22859831.E-prostanoid2ReceptorsDampenMastCellDegranulationviacAMP/PKA-MediatedSuppressionofIgE-SependentSignaling.

Cat. No. 250928 Anti-HumanIgMAntibody
DongZX.etal.2013.WorldJGastroenterol. PMID23901222.Resistinmediatesthehepaticstellatecell phenotype.

Cat.No. 252237 Anti-RabbitIgGHRP-Conjugated
MengQ.etal.2014. TumourBiol. PMID25168370. HMGB1promotescellularproliferationandinvasion,suppressescellularapoptosisinosteosarcoma.

Cat.No. 252240 Anti-GoatIgGHRP-Conjugated
TanJ.etal.2011.TumourBiol. PMID 21874375.ExpressionandSignificanceofSix1andEzrininCervicalCancerTissue.
 

Contactour CustomerServiceDept forcomprehensivelisting,availability,andnewproductstobereleased.
 
ABBIOTEC isabiotechcompanyfoundedin2007in SanDiego,California,arenownedworld-classLifeSciencesclusterthathostsmorethan1,500researchlabsandbiotechcompanies.Wearefocusedondeveloping,producingandcommercializing nextgenerationantibodies anduniquereagents fortheLifeSciencesresearchcommunityincludingthediagnosticandpharmaceuticalmarkets.
ABBIOTECoffers yearsofexpertise makingantibodiesandassaykitsforcatalogproductstothescientificandbiotechcommunity.Withourrecently upgradedanimalfacility,weofferpolyclonalantibodyproduction insmallandlargeanimals(rabbits,goats,llama)aswellasmonoclonalantibodyproduction (miceandrats).Because peptidechemistry isanessentialtooltoBIOLOGy,weoffer custompeptidesynthesisservices forresearch gradepeptides.

FOLLOWUSONLINKEDIN -ABBIOTEChasanew LinkedInpage.Getthelatestnewsfromthescientificcommunity,discoverourproductsandservicesandlookforjob opportunities!

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