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TheactivationofthelectincomplementpathwaysinmicecannowbemeasuredwiththenewHycultBiotechAssaysHIT420and HIT421.
MouseClassicalComplementPathwayAssay (HIT420)
MouseLectinComplemementPathwayAssay (HIT421)
Theinnateimmunesystemisimportantinthefirstdefenseagainstforeignpathogens.Amajorcomponentofthisresponseisthecomplementsystem.ComplementactivationproceedsinasequentialfashionthroughtheproteolyticcleavageofaseriesofproteinsleADIngtothegenerationofactivatedproductsthatmediatevariousBIOLOGicalactivitiesthroughtheirinteractionwithspecificcellularreceptorsandotherserumproteins.Thethreepathways,designatedclassical,lectinandalternativepathway,convergeatacentralcomponentintoafinalcommonpathway.ThatisactivationofC3leadingtoformationofC3aandC3b.ThiscleavageactivatestheterminalcomplementpathwayleadingtoeventuallytheformationoftheterminalC5b-9complementcomplex(TCC).
ThecomplementpathwayassaysareanewalternativefortheAP50andCH50tests,thataremorecumbersomeandvariableduetothefactthatthetestisperformedwithsheeperythrocytes.ThemousepathwayAssays are easytouseandspecific forits pathwaybymakinguseofacombinationofspecializedcoatingsandbuffers.Complementdeficienciesorotherdefectsinthecomplementsystemcaneasilybescreenedbyrunning thisassay.