| Description | TheMyoglobinELISAisintendedforthequantitativedeterminationofmyoglobininhumanserum.Myoglobin,ahemeproteinwithamolecularweightofapproximately17,500Daltonsisfoundinbothcardiacandskeletalmuscle.Damagetoeithertypeofmusclefollowingconditionssuchastrauma,ischemia,anddiseasesthatcausemyopathy,isassociatedwiththereleaseofmyoglobinintoserum.Specifically,followingcardiacnecrosisassociatedwithmyocardialinfarction(MI),myoglobinisoneofthefirstMarkerstoriseabovenormallevels.Anumberofreportssuggestusingthemeasurementofmyoglobinasadiagnosticaidinrulingoutmyocardialinfarctionwithnegativepredictivevaluesofupto100%reportedatcertaintimeperiodsaftertheonsetofsymptoms.UnliketheothercardiacenzymessuchascreeatinekinaseandtheMBisoform(i.e.,CKandCK/MB)whichdonotreachserumlevelsuntilseveralhourspost-infarction(approx.19hours),myoglobinlevelscanbeexpectedtopeakwithin6to9hours. |
|---|---|
| Species | Human |
| SampleType | Serum |
| Name | MyoglobinELISA |
|---|---|
| ShortDescription | TheMyoglobinELISAisintendedforthequantitativedeterminationofmyoglobininhumanserum.Myoglobin,ahemeproteinwithamolecularweightofapproximately17,500Daltonsisfoundinbothcardiacandskeletalmuscle.Damagetoeithertypeofmusclefollowingconditionssuchastrauma,ischemia,anddiseasesthatcausemyopathy,isassociatedwiththereleaseofmyoglobinintoserum.Specifically,followingcardiacnecrosisassociatedwithmyocardialinfarction(MI),myoglobinisoneofthefirstmarkerstoriseabovenormallevels.Anumberofreportssuggestusingthemeasurementofmyoglobinasadiagnosticaidinrulingoutmyocardialinfarctionwithnegativepredictivevaluesofupto100%reportedatcertaintimeperiodsaftertheonsetofsymptoms.UnliketheothercardiacenzymessuchascreeatinekinaseandtheMBisoform(i.e.,CKandCK/MB)whichdonotreachserumlevelsuntilseveralhourspost-infarction(approx.19hours),myoglobinlevelscanbeexpectedtopeakwithin6to9hours. |
| Reactivity | Human |