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| Name | Affinity Purified Anti-HUMAN IgG F(ab')2 (RABBIT) (Min X MOUSE Serum Proteins) |
|---|---|
| Related Product Names | Affinity Purified anti-Human IgG F(ab)2 [Rabbit] Minimum Cross Reactivity to Mouse Serum Proteins; N/A Affinity Purified Anti-HUMAN IgG F(ab')2 (RABBIT) (Min X MOUSE Serum Proteins)IGHG1 |
| Clone | Polyclonal |
| Immunogen | Anti-Human IgG F(ab')2 fragment was produced in rabbit by repeated immunization with human IgG F(ab')2 fragment. |
| Product Format | Liquid (sterile filtered) |
| Swiss Prot Number | P01857 |
| Specificity | Anti-Human IgG F(ab')2 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgG, Human IgG F(ab')2 and Human Serum. No reaction was observed against Human IgG F(c) or Mouse Serum Proteins. |
| Applications | WB, ELISA, IHC, Fluorescence Microscopy, FACS, Flow Cytometry (FACS), ChIP, Neutralization |
| Application Info | ELISA: 1:350,000 WB: 1:3,000 - 1:15,000 IHC: 1:1,000 - 1:5,000 Anti-Human IgG F(ab')2 antibody is suitable for western blotting, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below. |
| Format | Liquid (sterile filtered) |
| Reconstitution and Storage | Store vial at 4 C prior to opening. This product is stable for several weeks at 4 C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening product. |
| Reactivity | Human IgG F(ab')2 |
| Storage | -20C or Colder |
| Lead Time | Domestic: within 1-2 weeks delivery International: 1-2 weeks |
| Intended Use | Research Use Only |
| Key Reference | Janeway, Jr., Travers, Walport, and Shlomchik. "The Immune System in Health and Disease." Immunobiology, 5th Edition: Garland Science: 2001. |
| Additional Information | Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| :: | Label: Unconjugated Preservative: 0.01% (w/v) Sodium Azide Stabilizer: None |
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