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GenWay/pOET Sequencing Primers/GWB200100/2 x 100 μl

Description:

ThepOETsequencingprimersannealtotheupstreamanddownstreamregionsofthemultiplecloningsite.TheycanbeusedtosequenceacrosstheMCSoramplifyaninsertwithinitbyPCR.Theseprimersaresingle-strandedoligonucleotideswith5­hydroxyland3-hydroxylends.Allprimersaresuppliedas10µMaqueous solutions. 

pOET-Fforwardsequencingprimer,32-mer

5-CAAACTAATATCACAAACTGGAAATGTCTATC-3

Tm(50mMNa+):                                 52°C

MolecularWeight(µg/:µmol):           9769.4

 

pOET-Rreversesequencingprimer,29-mer

5-TACAACAATTGTCTGTAAATCAACAACGC-3

Tm(50mMNa+):                                  51°C

MolecularWeight(µg/:µmol):           8838.8

 

AdditionalInformation:


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NamepOETSequencingPrimers
ConcentrationLotSpecific
Storage-20C
IntendedUseResearchUseOnly