GenWay/Adiponectin (mouse) Elisa Assay Kit/GWBAXR240/1 x 96 assays

Description:

Description:ADIposetissuesecretesanumberofBIOLOGicallyactivesolublefactors(collectivelynamedadipocytokines)thatregulateglucoseandfattyacidmetabolism.Measurementofserumadiponectinlevelsgivesusimportantinformationontheroleofadiponectininregulationofglucoseand/orlipidmetabolism.ThisMouseAdiponectinELISAKitisanenzyme-linkedimmunosorbentassay(ELISA)forquantitativedeterminationofadiponectininmouseserum,plasmaorvarioustissueorcellculturesupernatants.Intheassay,monoclonalantibodyspecificformouseadiponectinhasbeenpre-coatedonto96wellmicroplate.StandardsandsamplesarePipettedintothewellsandadiponectinpresentisboundbyimmobilizedantibody.Theboundadiponectinisthencapturedbyanti-mouseadiponectinpolyclonalantibody.WithHRPconjugatedanti-rabbitIgGandaHRPsubstrate,thecolorsdevelopedinproportiontotheboundadiponectin,canbeeasilymeasuredbyElisaplatereader.
Summary:
-Detectionmethod-Absorbance(450nm)
-Sampletype-CellandTissueculturesupernatants,urine,plasmaandserum,aswellasmanyotherbiologicalfluids
-Speciesreactivity-Mammalian
-Applications-ThismouseAdiponectinELISAKitisanenzyme-linkedimmunosorbentassay(ELISA)forquantitativedeterminationofadiponectininmouseserum,plasmaorvarioustissueorcellculturesupernatants
Kitcomponents:
-Antibodycoated96-wellplate
-5XWashconcentrate,100ml
-5XDiluent,50ml
-Secondaryantibody,12ml
-100XDetector,150µl
-Standard,recombinantmouseadiponectin(16ng),1vial,lyophilized
-QCsample=positivecontrolhaving16-22µg/mlofmouseserumadiponectin
-SubstrateI,6ml
-SubstrateII,6ml
-Stopsolution,12ml
FeaturesandBenefits:
-Simpleprocedure
-Fastandconvenient
ShippingConditions:Gelpack

AdditionalInformation:

Name	Adiponectin(mouse)ElisaAssayKit
RelatedProductNames	AdiponectinElisaAssayKit,adiponectin,adiponectinEIA,mouseadiponectin,diponectinelisa
Format	AssayKit
Storage	+4C
IntendedUse	ResearchUseOnly