GenWay/Annexin VCy3 Apoptosis Kit Plus 25 assays/GWBAXR154/25 assays

Description:

Description:Theassayisbasedontheobservationthatsoonafterinitiatingapoptosis,cellstranslocatethemembranephospholipidphosphatidylserine(PS)fromtheinnerfaceoftheplasmamembranetothecellsurface.Onceonthecellsurface,PScaneasilybedetectedbystainingwithafluorescentconjugateofAnnexinV,aproteinthathasastrongnaturalaffinityforPS.Theone-stepstainingproceduretakesonly10minutes.Inaddition,theassaycanbedirectlyperformedonlivecells,withouttheneedoffixation.TheAnnexinV-Cy3ApoptosisDetectionKitPlusincludesannexinV-Cy3,SYTOXgreendye,andbindingbuffer.TheSYTOXgreendyeisimpermeanttolivecellsandapoptoticcells,butstainsnecroticcellswithintensegreenfluorescencebybindingtocellularnucleicacids.AfterstainingacellpopulationwithannexinV-Cy3andSYTOXGreendyeintheprovidedbindingbuffer,apoptoticcellsshowredfluorescence,deadcellsshowgreenfluorescenceandlivecellsshowlittleornofluorescence.ThesepopulationscaneasilybedistinguishedbyFluorescencemicroscopyusingFITCandrhodaminefiltersorbyflowcytometryusingtheFL1channel(Ex.488nm/Em.530nm)forSYTOXGreendyeandFL2channelforAnnexinV-Cy3(Ex.543nm/Em.570nm).
Summary:
-Detectionmethod-flowcytometryusingFL1channelforSYTOXGreendye(Ex=488nm;Em=530nm)andFL2channelforAnnexinV-Cy3(Ex=543nm;Em=570nm).
-Sampletype-Livecells
-Speciesreactivity-Mammalian
-Kitsize-Convenientsizes(25,100and400assays)
-Applications-Detectingapoptosisinlivingcellsbyflowcytometryorfluorescencemicroscopy.Cy3showsbrighterredfluorescence.
Kitcomponents:
-AnnexinV-Cy3
-SYTOXGreenDye
-BindingBuffer
FeaturesandBenefits:
-Simpleone-stepprocedure;takesonly10minutes
-Fastandconvenient
-Apoptoticcellsshowredfluorescence,deadcellsshowgreenfluorescenceandlivecellsshowlittleornofluorescence.
ShippingConditions:Gelpack

AdditionalInformation:

Name	AnnexinV-Cy3ApoptosisKitPlus-25assays
RelatedProductNames	AnnexinV-Cy3ApoptosisKitPlus,AnnexinV-Cy3ApoptosisKit,AnnexinV,AnnexinV-Cy3,AnnexinVApoptosis,AnnexinVAssay,AnnexinV,AnnexinV,Annexin-V,Annexin-5,Annexin5,Annexin5
Format	AssayKit
Storage	+4C
IntendedUse	ResearchUseOnly