GenWay/RNF20 Active Human Recombinant Protein/GWBPSA6BC/20µg

Description:

Description:Humanringfingerprotein20,orRNF20(GenBankAccessionNo.NM_019592),fulllength,a.a.2-975(end)withN-terminalHis-Flagtag,MW=115kDa,expressedinaBaculovirusinfectedSf9cellexpressionsystem.AssayConditions:50µlreactionmix(4mMATP,5mMMgCl2,1mMDTT,E1(0.5µg),E2(2.8µg),Biotin-Ubiquitin(0.5µg)andRNF20in50mMTrispH7.4)incubatefor30minat37°C.Thenaddtostreptavidincoatedplatesandincubatefor30minat37°C.AddantibodyagainstRNF20,incubate1hour.Finally,incubate1hourwiththeHRP-labeledsecondaryantibodyFormulatedin:40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,80ng/μlFlagpeptide,20%glyceroland3mMDTT.Stability:>6monthsat-80°CReferences:1.Shiloh,Y.,etal.,FEBSLett.2011Sep.16;585(18):2795-2802.2.Shema,E.,etal.Mol.Cell.2011May20;42(4):477-488

Host:baculovirus

Tag:N-terminalHis-Flagtag

GINum:NM_019592

MW:115kDa

Format:aqueousbuffersolution

Formula:40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,80ng/μlFlagpeptide,20%glyceroland3mMDTT

ScientificCategory:UbiquitinE3ligase

Species:human

AdditionalInformation:

Name	RNF20ActiveHumanRecombinantProtein
RelatedProductNames	RNF20ActiveHumanRecombinantProtein
MolecularWeight	115kDa
Purity	40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,80ng/μlFlagpeptide,20%glyceroland3mMDTT
Source	baculovirus
GINumber	NM_019592
Format	AqueousBufferSolution
Reactivity	Human
Storage	-80°C
IntendedUse	ResearchUseOnly