GenWay/Mouse IFNgamma ELISA Kit/GWBSKR076/96 Tests

Description:

ShortDescription:ThisproductisaSandwichELISAwithadetectionmethodofColorimetric450nm.TheMurineIFN-gammaELISAKitallowsforthedetectionandquantificationofendogenouslevelsofnaturaland/orrecombinantMurineIFN-gammaproteinswithintherangeof23-1500pg/ml.TheMurineIFN-gammaELISAiscapableofrecognizingbothrecombinantandnaturallyproducedMurineIFN-gammaproteins.Theantigenslistedbelowweretestedat50ng/mlandexhibited100%crossreactivity.•RatIFN-gammaTheantigenslistedbelowweretestedat50ng/mlanddidnotexhibitsignificantcrossreactivityorinterference.•HumanIFN-alpha,IFN-beta,IFN-gamma,IFN-?1,IFN-?2,IL-10,IL-18•MurineIFN-?2,IL-10,IL-12,IL-18•RatIL-10

AdditionalInformation:

Name	MouseIFN-gammaELISAKit
RelatedProductNames	Interferongamma
GeneSymbol	IFNG
GeneFamily	IFN
GeneFullname	interferongamma
Geneid	15978
AliasSymbols	Ifg,IFN-g,IFN-gamma,Interferongamma
ProteinName	Interferongamma
DescriptionofTarget	ThisgeneencodesasolublecytokinethatisamemberofthetypeIIinterferonclass.Theencodedproteinissecretedbycellsofboththeinnateandadaptiveimmunesystems.Theactiveproteinisahomodimerthatbindstotheinterferongammareceptorw
SwissprotID	P01580
ProteinAccessionNum	NP_032363.1
NucleotideAccessionNum	NM_008337.4
SpeciesReactivity	Mouse
AssayInfo	QuantitativeColorimentricSandwichELISA
Sensitivity	23pg/mL
KitRange	23-1500pg/mL
StandardRange	23-1500pg/ml
SampleType	Celllysates,seraandplasma
ProductFormat	1x96-WellPlateor12x8-WellStrips
Specimen/Volumes	RefertoManual
SwissProtNumber	P01580
Specificity	TheMurineIFN-γELISAiscapableofrecognizingbothrecombinantandnaturallyproducedMurineIFN-γproteins.Theantigenslistedbelowweretestedat50ng/mlandexhibited100%crossreactivity. •Rat:IFN-γ Theantigenslistedbelowweretestedat50ng/mlanddidnotexhibitsignificantcrossreactivityorinterference. •Human:IFN-α,IFN-β,IFN-γ,IFN-λ1,IFN-λ2,IL-10,IL-18 •Murine:IFN-λ2,IL-10,IL-12,IL-18 •Rat:IL-10
Applications	ELISA
GINumber	15978
Format	ELISAKit.Refertomanualforspecificcomponentformats
Stability	6months
ReconstitutionandStorage	4C/6Months
Reactivity	Mouse
KitSize	12x8-WellMicrostrips
KitPrinciple	TheAvivaMurineIFN-γELISAKitcontainsthecomponentsnecessaryforquantitativedeterminationofnaturalorrecombinantmIFN-γconcentrationswithinanyexperimentalsampleincludingcelllysates,serumandplasma.Thisparticularimmunoassayutilizesthequantitativetechniqueofa"Sandwich"Enzyme-LinkedImmunosorbentAssay(ELISA)wherethetargetprotein(antigen)isboundina"sandwich"formatbytheprimarycaptureantibodiescoatedtoeachwell-bottomandthesecondarydetectionantibodiesaddedsubsequentlybytheinvestigator.ThecaptureantibodiescoatedtothebottomofeachwellarespecificforaparticularepitopeontheMurineIFN-γcytokinewhiletheuser-addeddetectionantibodiesbindtoepitopesonthecapturedtargetprotein.Amideachstepoftheprocedure,aseriesofwashstepsmustbeperformedtoensuretheeliminationofnon-specificbindingbetweenproteinstootherproteinsortothesolidphase.Afterincubationand"sandwiching"ofthetargetantigen,aperoxidaseenzymeisconjugatedtotheconstantheavychainofthesecondaryantibody(eithercovalentlyorviaAvidin/Streptavidin-Biotininteractions),allowingforacolorimetricreactiontoensueuponsubstrateaddition.WhenthesubstrateTMB(3,3’,5,5’-Tetramethylbenzidine)isadded,thereactioncatalyzedbyperoxidaseyieldsabluecolorthatisrepresentativeoftheantigenconcentration.Uponsufficientcolordevelopment,thereactioncanbeterminatedthroughadditionofStopSolution(2NSulfuricAcid)wherethecolorofthesolutionwillturnyellow.Theabsorbanceofeachwellcanthenbereadbyaspectrophotometer,allowingforgenerationofastandardcurveandsubsequentdeterminationofproteinconcentration.
KitComponent	Components Amount CaptureAntibodyCoatedMicroplate 96Wells(12x8) Ready-to-UseStreptavidin-HRP 12mL Ready-to-UseSubstrate 12mL StopSolution 12mL WashBuffer(15x) 50mL ProteinStandardDiluent 12mL SampleDiluent 12mL DetectionAntibodyDiluent 12mL Biotin-LabeledDetectionAntibody Lyophilized ProteinStandard Lyophilized(100ng)
Storage	4°C/6Months
LeadTime	Domestic:within1weekdelivery International:1week
IntendedUse	ResearchUseOnly