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GenWay/Guinea Pig IgA ELISA /GWB374Z25/96 well

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NameGuineaPigIgAELISA
RelatedProductNamesIgA,ImmunoglobulinA,GuineaPigIgA,GuineaPigImmunoglobulinA,GuineaPigImmunoglobulinAELISA
AliasSymbolsIGHA1,IGHA2,IgA,Igalpha1chainCregion,Igalpha2chainCregion
ProteinNameIgA
SpeciesReactivityGuineaPig
AssayInfoAssayMethodology:QuantitativeSandwichELISA
Sensitivity1.212ng/ml
KitRange6.25ng/ml-200ng/ml
HomologyGuineaPig
StandardRange200ng/ml-6.25ng/ml
SampleTypeSerum,plasmaandotherbiologicalfluids
ProductFormatInSolution
Specimen/VolumesReferToManual
ConcentrationLotSpecific
ApplicationsELISA
ReconstitutionandStorage1.DILUENT
The5XDiluentConcentrateisstableuntiltheexpira-tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionsshouldbestoredat4-8C.
2.WASHSOLUTION
The20XWashSolutionConcentrateisstableuntiltheexpira¬tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionscanbestoredatroomtemperature(1625C)orat4-8C.
3.ENZYME-ANTIBODYCONJUGATE
Undilutedhorseradishperoxidaseanti-IgAconjugateshouldbestoredat4-8Canddilutedimmediatelypriortouse.Theworkingconjugatesolutionisstableforupto8hours.
4.CHROMOGEN-SUBSTRATESOLUTION
TheSubstrateSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate.
5.STOPSOLUTION
TheStopSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate.
6.ANTI-GUINEAPIGIgAELISAMICROPLATE
Anti-GuineaPigIgAcoatedwellsarestableuntiltheexpirationdate,andshouldbestoredat4-8Cinsealedfoilpouchwithdesiccantpack.
7.GUINEAPIGIgASTANDARDS
AliquotGuineaPigIgAcalibratorandstorethemfrozen,avoidrepeatedfreeze-thawcycles.Forstoragelongerthan14days,keepfrozenuntiltheexpirationdate.Storageoflessthan14dayscanbe4C.Theworkingstandardsolutionsshouldbepreparedimmediatelypriortouseandarestableforupto8hours.
ReactivityGuineapig
KitSize96well
KitPrincipleGenwayBiotechIgAELISAKit(GuineaPig)(GWB-374Z25)isbasedonstandardsandwichenzyme-linkedimmuno-sorbentassaytechnology.AnantibodyforIgAhasbeenpre-coatedontoa96-wellplate(12x8WellStrips).Standardsortestsamplesareaddedtothewells,incubatedandwashedtoremoveunboundproteins.AnAnti-IgA-HRPconjugateddetectorantibodyisthenadded,incubatedandunboundconjugateiswashedaway.AnenzymaticreactionisproducedthroughtheadditionofTMBsubstratewhichiscatalyzedbyHRPgeneratingabluecolorproductthatchangestoyellowafteraddingacidicstopsolution.Thedensityofyellowcolorationreadbyabsorbanceat450nmisquantitativelyproportionaltotheamountofsampleIgAcapturedinthewell.
KitComponent
ComponentAmount
Anti-GuineaPigIgAMicroplate96Wells(12x8WellStrips)
20XDiluentConcentrate50mL
20XWashSolution50mL
100XEnzyme-AntibodyConjugate150uL
Chromogen-SubstrateSolution12mL
StopSolution12mL
GuineaPigIgACalibrator1
KitDuration50Minutes
StorageRefertokitinstructionsforcomponentstorage
LeadTimeDomestic:within1weekdelivery International:1week
IntendedUseResearchUseOnly
AdditionalInformationIntendedUse:TheIgAtestkitsareahighlysensitivetwo-siteenzymelinkedimmunoassay(ELISA)formeasuringIgAinbiologicalsamplesofGuineaPigs.
::Reagents:
1.DILUENTCONCENTRATE(RunningBuffer)
Onebottlecontaining50mlofa5Xconcentrateddiluentrunningbuffer.
2.WASHSOLUTIONCONCENTRATE
Onebottlecontaining50mlofa20Xconcentratedwashsolution.
3.ENZYME-ANTIBODYCONJUGATE100X
Onevialcontaining150ulofaffinitypurifiedanti-GuineaPigIgAantibodyconjugatedwithhorseradishperoxidaseinastabilizingbuffer.
4.CHROMOGEN-SUBSTRATESOLUTION
Onevialcontaining12mLof3,3’,5,5’-tetramethybenzidine(TMB)andhydrogenperoxideincitricacidbufferatpH3.3.
5.STOPSOLUTION
Onevialcontaining12ml0.3Msulfuricacid.
WARNING:Avoidcontactwithskin.
6.ANTI-GUINEAPIGIgAELISAMICROPLATE
Twelveremovableeight(8)wellmicrowellstripsinwellholderframe.Eachwelliscoatedwithaffinitypurifiedanti-GuineaPigIgA.
7.GUINEAPIGIgACalibrator
OnevialcontainingGuineaPigIgAcalibrator.