GenWay/Mycoplasma Removal Agent Reagent/GWBQ00261/5 ml

Description:

Description:MRAisveryeffectiveatremovingmycoplasmafrominfectedcellcultures.Itshowsstronganti-mycoplasma_x000D_activityagainstmanytypesofmycoplasmaincluding

AdditionalInformation:

Name	MycoplasmaRemovalAgentReagent
RelatedProductNames	MYCOPLASMAREMOVALAGENTReagent,TissueCulture,MYCOPLASMAREMOVALAGENT
DescriptionofTarget	MYCOPLASMAREMOVALAGENT
Applications	TissueCulture
Format	Kit
Stability	5yearsfromdateofdespatch.
ReconstitutionandStorage	Storeatroomtemperature. Thisproductshouldbestoredundiluted. Thisproductisphotosensitiveandshouldbeprotectedfromlight.
Storage	Storeatroomtemperature. Thisproductshouldbestoredundiluted. Thisproductisphotosensitiveandshouldbeprotectedfromlight.
LeadTime	Domestic:within2-3weeksdelivery International:2-3weeks
IntendedUse	ResearchUseOnly
KeyReference	1.Nakai,N.etal.(2000)DetectionandeliminationofcontaminatingmicroorganismsinTransplantableTumorsandCellLines.Exp.Anim.49:309-313.
AdditionalInformation	ApproxProteinConc:50ug/ml
::	IntendedUse:MRAisveryeffectiveatremovingmycoplasmafrominfectedcellcultures.Itshowsstronganti-mycoplasmaactivityagainstmanytypesofmycoplasmaincludingMycoplasmaorale,M.arginini,M.hyorhinisandAcholeplasmalaidlawii. MRAisalsosuitableforuseaftertheremovalofmycoplasma,topreventrecontaminationoftheculturewiththeoriginalmycoplasma,atpreventativedoses. Thisproductcanalsobeusedtopreventinitialinfectionofcellsinculturebymycoplasma. MRAisnontoxic,andwillnotinterferewiththeviabilityorfunctionofcellsinculture.ItshouldbeemphasisedthatMRAshouldnotbeusedasasubstituteforgoodcellculturetechniques.
::	InstructionsForUse: MRAisveryeasytouse,simplyrequiringincubationforaweekafteradditiontocellculturescontaminatedbymycoplasma. IndicationsforUse: 1.AddMRAtocellculturescontaminatedbymycoplasmaataconcentrationof0.5ug/mlandincubateforaweek. 2.Formediareplacementorculturetransfer(passage),useamediumcontainingMRAatthissameconcentration. 3.TransferthecellculturesseveraltimeswithoutMRAandconfirmthatregrowthofthecontaminatingmycoplasmahasnotoccurred. Ifthereisaconcernaboutthepresenceofmycoplasmainserumortrypsin,MRAcanbeaddedtothemediaataconcentrationof0.5ug/mltopreventcontaminationofthecellculturesexposedtotheseproducts. N.B.Therecommendedconcentrationforuseis0.5ug/ml.TheMRAconcentrationmayberaisedupto1ug/mlonlywhentherecommendedconcentrationisineffectiveinremovingthemycoplasma. ThecytotoxicityofMRAislowandcelltoxicityisrarewhenusedattherecommendedconcentration.Forspecificfunctionofanycell,however,itisrecommendedthattheretentionofdesiredcellularcharacteristicsbeconfirmedaftertreatment.