GenWay/HDAC2 Fluorogenic Assay Kit/GWBPS3366/96rxns

Description:

Description:TheFluorogenicHDAC2AssayKitisacompleteassaysystemdesignedtomeasurehistonedeacetylase2(HDAC2)activityforscreeningandprofilingapplications.Itcomesinaconvenient96-wellformat,withallthereagentsnecessaryfor100uluorescentHDAC2activitymeasurements.Inaddition,thekitincludespurifiedHDAC2enzymeandapotentHDACinhibitor,TrichostatinA,foruseasapositiveandnegativecontrol.TheFluorogenicHDAC2AssayKitisbasedonauniquefluorogenicsubstrateanddevelopercombination.Thisassaymethodeliminatesdealingwiththeradioactivity,extraction,andchromatographyaspectsoftraditionalassays.Usingthiskit,onlytwosimplestepsonamicrotiterplateareneededtoanalyzetheHDACactivitylevel.First,theHDACfluorometricsubstrate,containinganacetylatedlysinesidechain,isincubatedwithpurifiedHDAC2.ThedeacetylationsensitizesthesubstratesosubsequenttreatmentwiththeLysineDeveloperproducesafluorophorethatcanthenbemeasuredusingafluorescencereader.

Format:assaykit

ScientificCategory:DeacetylaseAssay

AdditionalInformation:

Name	HDAC2FluorogenicAssayKit
RelatedProductNames	histonedeacteylase,HDAC2
Purity	NA
Format	AssayKit
Storage	Mixedstorage
IntendedUse	ResearchUseOnly